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Difference between revisions of "MiPNet17.04 CitrateSynthase"

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{{OROBOROS header page name}}
{{Publication
{{Publication
|title=Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger E. Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 17.04.
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.
|info=[http://www.oroboros.at/?Protocols_CS MiPNet17.04]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/4/40/MiPNet17.04_CitrateSynthase.pdf|Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet17.04_CitrateSynthase.pdf Versions]
|authors=Eigentler A, Draxl A, Wiethuechter A, Kuznetsov AV, Lassnig B, Gnaiger E
|authors=Oroboros
|year=*
|year=2020-04-18
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al 1970; Willimas et al 1986; Hood et al 1989), although this role of CS has been questioned in developmental (Drahota et al 2004), age-related studies (Marin-Garcia et al 1998), physical training ([[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta et al 2011]]) and cardiac disease studies ([[Lemieux 2011 Int J Biochem Cell Biol|Lemieux et al 2011]]). Proliferation of mitochondria in pathological states is sometimes associated with an increase in CS activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein or per mt-respiratory capacity ([[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]). Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications ([[Kuznetsov 2002 Analyt Biochem|Kuznetsov et al 2002]]; [[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]; [[Huetter 2004 Biochem J|Hütter el al 2004]]).
|abstract=Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: [[Citrate synthase]] a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.
|keywords=Enzymes
 
|mipnetlab=AT_Innsbruck_OROBOROS
Version 04: New Section '''6. Normalization of respiratory flux for CS activity'''
|discipline=Mitochondrial Physiology
|keywords=Normalization of rate
|articletype=Protocol; Manual, MiPNet-online Publication
|mipnetlab=AT Innsbruck Oroboros
}}
}}
{{MiPNet pdf page linking to MitoPedia}}
== Author contributions ==
:::: This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project ''MitoCom Tyrol'', funded by the Tyrolian Government and the European Regional Development Fund (ERDF).
:::: Last updates: contribution to H2020 Project NextGen-O2k.
{{Template:Keywords: Normalization}}
{{Labeling
{{Labeling
|instruments=Oxygraph-2k, Protocol, MiPNet-Publication
|area=Respiration, Instruments;methods
|preparations=Enzyme
|preparations=Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme
|topics=Mitochondrial Biogenesis; Mitochondrial Density
|enzymes=Marker enzyme
|additional=Enzymes
|instruments=Oxygraph-2k, O2k-Protocol
|discipline=Mitochondrial Physiology
|additional=Mt- and marker-enzymes, Normalization of rate
|articletype=Protocol; Manual, MiPNet-online Publication
}}
}}
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Latest revision as of 14:56, 1 February 2021

                



MiPNet17.04 CitrateSynthase

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O2k-Protocols
Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.

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Oroboros (2020-04-18) Mitochondr Physiol Network

Abstract: Eigentler A, Draxl A, Gnaiger E (2020) Laboratory protocol: Citrate synthase a mitochondrial marker enzyme. Mitochondr Physiol Network 17.04(04):1-12.

Version 04: New Section 6. Normalization of respiratory flux for CS activity Keywords: Normalization of rate

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Author contributions

This MiPNet Protocol was originally prepared by Kuznetsov AV, Lassing B, Gnaiger E as MiPNet08.14 (2003) and further edited by Wiethüchter A (2011). MiPNet17.04 (2012 version) was edited by Eigentler A, Draxl A, Gnaiger E; contribution to K-Regio project MitoCom Tyrol, funded by the Tyrolian Government and the European Regional Development Fund (ERDF).
Last updates: contribution to H2020 Project NextGen-O2k.


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Labels: MiParea: Respiration, Instruments;methods 



Preparation: Permeabilized cells, Permeabilized tissue, Homogenate, Isolated mitochondria, Enzyme  Enzyme: Marker enzyme 


HRR: Oxygraph-2k, O2k-Protocol 

Mt- and marker-enzymes, Normalization of rate