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Difference between revisions of "MiPNet17.04 CitrateSynthase"

From Bioblast
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{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. '''Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger E (2012) Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 17.04.''' [[Media:MiPNet17.04 CitrateSynthase.pdf|»Bioblast pdf«]]
|title=[[Image:O2k-Protocols.jpg|right|80px|link=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols]] Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. [[Media:MiPNet17.04 CitrateSynthase.pdf|»Bioblast pdf«]]
|info=[http://www.bioblast.at/index.php/File:MiPNet17.04_CitrateSynthase.pdf Versions]
|info=[http://www.bioblast.at/index.php/File:MiPNet17.04_CitrateSynthase.pdf Versions]
|authors=OROBOROS
|authors=OROBOROS
|year=2012-06-28
|year=2015-06-18
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract=Citrate synthase (E.c. 4.1.3.7) is a pace-maker enzyme in the Krebs cycle (citric acid cycle). Citrate synthase, CS, has a molecular weight of 51,709 Da, with gene map locus 12q13.2-q13.3. CS is localized in the mitochondrial matrix, but is nuclear encoded, synthesized on cytoplasmic ribosomes and transported into the mitochondrial matrix. CS, therefore, is commonly used as a quantitative marker enzyme for the content of intact mitochondria (Holloszy et al 1970; Willimas et al 1986; Hood et al 1989), although this role of CS has been questioned in developmental (Drahota et al 2004), age-related studies (Marin-Garcia et al 1998), physical training ([[Pesta 2011 Am J Physiol Regul Integr Comp Physiol|Pesta et al 2011]]) and cardiac disease studies ([[Lemieux 2011 Int J Biochem Cell Biol|Lemieux et al 2011]]). Proliferation of mitochondria in pathological states is sometimes associated with an increase in CS activity per cell, but CS activity in a specific tissue is frequently constant when expressed per mitochondrial protein or per mt-respiratory capacity ([[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]). Mitochondrial, cellular or tissue respiration, therefore, may be expressed per CS activity for specific applications ([[Kuznetsov 2002 Analyt Biochem|Kuznetsov et al 2002]]; [[Renner 2003 Biochim Biophys Acta|Renner et al 2003]]; [[Huetter 2004 Biochem J|Hütter el al 2004]]).
|abstract=Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger  E (2012) Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 17.04.
 
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
:>> Product: [http://www.oroboros.at/?oxygraph OROBOROS O2k], [[O2k-Catalogue_OROBOROS| O2k-Catalogue]]
|mipnetlab=AT Innsbruck OROBOROS
|mipnetlab=AT Innsbruck OROBOROS

Revision as of 14:06, 18 June 2015

Publications in the MiPMap
O2k-Protocols
Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. »Bioblast pdf«

» Versions

OROBOROS (2015-06-18) Mitochondr Physiol Network

Abstract: Eigentler A, Draxl A, Wiethüchter A, Kuznetsov AV, Lassnig B, Gnaiger E (2012) Laboratory protocol: Citrate synthase. Mitochondrial marker enzyme. Mitochondr Physiol Network 17.04.

>> Product: OROBOROS O2k, O2k-Catalogue


O2k-Network Lab: AT Innsbruck OROBOROS


Labels: MiParea: Instruments;methods, mt-Biogenesis;mt-density 



Preparation: Enzyme  Enzyme: Marker enzyme 


HRR: Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

Mt- and marker-enzymes 

Further information

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