Difference between revisions of "MiPNet17.15 PBI-Shredder Mouse-heart-brain-liver"
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{{Publication | {{Publication | ||
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. | |title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. | ||
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/c/c9/MiPNet17.15_PBI-Shredder_Mouse-heart-brain-liver.pdf |Bioblast pdf]] | |info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/c/c9/MiPNet17.15_PBI-Shredder_Mouse-heart-brain-liver.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet17.15_PBI-Shredder_Mouse-heart-brain-liver.pdf Versions] | ||
|authors=Oroboros | |authors=Oroboros | ||
|year=2015-06-16 | |year=2015-06-16 | ||
|journal=Mitochondr Physiol Network | |journal=Mitochondr Physiol Network | ||
|abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6.''' | |abstract='''Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6.''' | ||
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR. | A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR. | ||
Revision as of 10:07, 11 January 2018
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Oroboros (2015-06-16) Mitochondr Physiol Network
Abstract: Eigentler A, Fontana-Ayoub M, Gnaiger E (2015) Tissue homogenates for diagnosis of mitochondrial respiratory function: Mouse heart, brain and liver. Mitochondr Physiol Network 17.15(04):1-6.
A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of respirometric and fluorometric studies. These considerations provided the rationale for initiating a study with the PBI-Shredder, an auxiliary HRR-Tool providing a standardized approach to prepare homogenates of various tissues (e.g. heart, liver, brain) with high reproducibility of 100% mitochondrial yield and mitochondrial function as evaluated with HRR.
- >> O2k-Protocols: Overall contents
- >> Product: Oroboros O2k, O2k-Catalogue
• Keywords: Tissue homogenate, Shredder
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT Innsbruck MitoCom
Labels: MiParea: Respiration, Instruments;methods
Organism: Mouse
Tissue;cell: Heart, Nervous system, Liver
Preparation: Homogenate
Coupling state: LEAK, OXPHOS, ET
Pathway: N, S, NS
HRR: Oxygraph-2k, O2k-Manual, O2k-Protocol
Mt-Preparation, PBI-Shredder
