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MiPNet18.06 Amplex-Yeast

From Bioblast
Publications in the MiPMap
Fontana-Ayoub M, Fasching M, Eigentler A, Laner V, Gnaiger E (2013) O2k-Fluorometry: Amplex® UltraRed using freeze-dried baker’s yeast. Mitochondr Physiol Network 18.06.

» Open Access-MiPNet18.06.pdf; Versions

O2k-Protocols contents

OROBOROS (2013) Mitochondr Physiol Network

Abstract: O2k-Protocol for Amplex® UltraRed using freeze-dried baker’s yeast

Respiration and hydrogen peroxide production of yeast was studied under hypoxic and hyperoxic conditions, and after stimulation of respiration by glucose using the O2k-Fluorescence LED2-Module. Extracellular H2O2-production by intact yeast cells was a pronounced function of environmental oxygen concentration (Fig. 1). Oxygen kinetics of respiration resembled closely but not fully that of isolated mitochondria, indicating that extracellular oxygen levels provide a good approximation of intracellular conditions, and intracellular oxygen gradients are small. Stimulation of respiration was pronounced with the extracellular substrates glucose and ethanol, and with uncoupler titrations. In contrast, these additions exerted a minor effect on extracellular H2O2 production (Fig. 2).

>> O2k-Protocols:Overall contents
>> Product: OROBOROS Oxygraph-2k, O2k-Catalogue


O2k-Network Lab: AT Innsbruck OROBOROS, AT Innsbruck MitoCom


Labels: MiParea: Respiration 


Organism: Saccharomyces cerevisiae 

Preparation: Intact cells 

Regulation: O2"O2" is not in the list (Aerobic glycolysis, ADP, ATP, ATP production, AMP, Calcium, Coupling efficiency;uncoupling, Cyt c, Flux control, Inhibitor, ...) of allowed values for the "Respiration and regulation" property., Uncoupler  Coupling state: ROUTINE, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property. 

HRR: Oxygraph-2k, Fluorometry"Fluorometry" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property., Protocol"Protocol" is not in the list (Oxygraph-2k, TIP2k, O2k-Fluorometer, pH, NO, TPP, Ca, O2k-Spectrophotometer, O2k-Manual, O2k-Protocol, ...) of allowed values for the "Instrument and method" property. 

O2k-MultiSensor 

Further information