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Difference between revisions of "MiPNet19.18D O2k-Series G and DatLab 6: Calibration"

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{{OROBOROS header page name}}
{{OROBOROS header page name}}
{{Publication
{{Publication
|title=[[Image:O2k-Manual.jpg|right|70px|link=O2k-Manual|O2k-Manual]] O2k-calibration by DatLab 6.
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-calibration by DatLab 6.
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/a/ab/MiPNet19.18D_O2k-Calibration.pdf |Bioblast pdf]]
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/a/ab/MiPNet19.18D_O2k-Calibration.pdf |Bioblast pdf]] »[http://www.bioblast.at/index.php/File:MiPNet19.18D_O2k-Calibration.pdf Versions]
|authors=Oroboros
|authors=Oroboros
|year=2015-08-08
|year=2016-08-24
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Gnaiger E (2016) Oxygen calibration by DatLab 6. Mitochondr Physiol Network 19.18(D04):39-48.''' » [http://www.bioblast.at/index.php/File:MiPNet19.18D_O2k-Calibration.pdf Versions]
|abstract=Gnaiger E (2016) Oxygen calibration by DatLab 6. Mitochondr Physiol Network 19.18(D04):01-12. »  
[[Image:MitoPedia.jpg|left|60px|link=http://www.bioblast.at/index.php/MitoPedia: DatLab|MitoPedia: DatLab]] [[O2 calibration - DatLab]]
{{MiPNet pdf page linking to MitoPedia}}
|keywords=
|mipnetlab=AT_Innsbruck_Oroboros
}}
 
{{Template:Keywords: Oxygen signal}}


'''O2k-Series G Manual (DatLab 6)''' [[Accuracy|Accurate]] calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the [[Ooroboros O2k]] at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the [[polarographic oxygen sensor]] (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate [[oxygen solubility]] for aqueous solutions for the conversion of partial [[oxygen pressure]] into oxygen concentration ([[MiPNet06.03]]). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.


:» O2k-SOP: [[MiPNet06.03 POS-calibration-SOP]]
:» Product: [[DatLab]], [[Oroboros O2k]], [[Oroboros O2k-Catalogue | O2k-Catalogue]]
|keywords=HRR, DatLab
|mipnetlab=AT_Innsbruck_Oroboros
}}
{{Labeling
{{Labeling
|area=Instruments;methods
|area=Instruments;methods
|instruments=Oxygraph-2k, O2k-Manual, O2k-Protocol
|instruments=Oxygraph-2k
|additional=O2k-Core, DatLab, DL6, DL6a7, SeriesG
|additional=O2k-Core, DatLab, DL6
}}
}}
== DatLab 7 ==
::::» '''DL-Protocol''' (DLP): [[MiPNet06.03_POS-calibration-SOP#Instrumental_O2_DLP|O2 calibration air]] - This DLP provides a guide through an oxygen calibration protocol (air calibration). Version 7 beta: check for or create a folder '''DL-Protocol''' on your PC and download the DLP file to this folder. DL-Protocols are functional in DatLab 7 only.
::::» '''Template''': [[Media:O2 calibration.xlsx |O2 calibration List]] - for quality control with a track record of O<sub>2</sub> calibrations.
{{Template:Technical support integrated}}
__TOC__
=== POS calibration ===
::: '''Static POS calibration''' = Two-point calibration of the [[polarographic oxygen sensor]].
:::: DatLab converts the [[raw signal]] to oxygen concentrations  by applying conversion factors determined during a POS calibration comprising an [[air calibration]] and a [[zero calibration]]. For each [[Gain (O2 channel) | gain]] a different set of calibration values is necessary. Frequently, assumed hardware problems turn out to be a simple calibration problem. This can easily be detected by observing the [[Raw signal]].
::: '''Dynamic POS calibration''' = Calibration of the [[MiPNet06.03 POS-calibration-SOP |response time of the POS]].
=== Related O2k-Protocols ===
::::» O2k-SOP: [[MiPNet06.03 POS-calibration-SOP]]
::::» [[MiPNet14.06 Instrumental O2 background]]
::::» [[DatLab]]
[[Category:OroboPedia]]

Latest revision as of 07:47, 29 January 2020

                



MiPNet19.18D O2k-Series G and DatLab 6: Calibration

Publications in the MiPMap
O2k-Protocols
O2k-calibration by DatLab 6.

» Bioblast pdf »Versions

Oroboros (2016-08-24) Mitochondr Physiol Network

Abstract: Gnaiger E (2016) Oxygen calibration by DatLab 6. Mitochondr Physiol Network 19.18(D04):01-12. »

MitoPedia: DatLab

O2 calibration - DatLab


O2k-technical support and open innovation
Open the pdf document above.
» Current O2k-series: NextGen-O2k Series XB and O2k Series J
» Current software versions DatLab 8.0: MitoPedia: DatLab


O2k-Network Lab: AT_Innsbruck_Oroboros



Questions.jpg


Click to expand or collaps



Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k 

O2k-Core, DatLab, DL6