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MiPNet19.18D O2k-Series G and DatLab 6: Calibration

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MiPNet19.18D O2k-Series G and DatLab 6: Calibration

Publications in the MiPMap
O2k-Manual
O2k-calibration by DatLab.

» Bioblast pdf

OROBOROS (2015-08-08) Mitochondr Physiol Network

Abstract: Gnaiger E (2016) Oxygen calibration by DatLab. Mitochondr Physiol Network 19.18(D04):39-48. » Versions

O2k-Manual: Accurate calibration of the oxygen sensor depends on (1) calibration solutions prepared at known oxygen partial pressures, as achieved in the O2k at defined temperature, continuously recorded total gas pressure (barometric pressure), and thermodynamic equilibrium between the gas and aqueous phase; (2) high stability of the signal of the polarographic oxygen sensor (POS), tested for sufficiently long periods of time; (3) linearity of signal output with oxygen pressure, achieved with the POS in the range between oxygen saturation and zero oxygen pressure; and (4) application of accurate oxygen solubility for aqueous solutions for the conversion of partial oxygen pressure into oxygen concentration (MiPNet06.03). The standard oxygen calibration procedure is described for O2k high-resolution respirometry with the automatic calibration routine of DatLab.

» O2k-SOP: MiPNet06.03 POS-Calibration-SOP
» Product: DatLab 7, O2k, O2k-Catalogue
» Template: O2k-calibration List

Keywords: HRR, DatLab

O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Instruments;methods 





HRR: Oxygraph-2k, O2k-Manual, O2k-Protocol 

O2k-Core, DatLab 




Template NextGen-O2k.jpg


MitoPedia O2k and high-resolution respirometry: O2k-Open Support 




POS calibration

Static POS calibration = Two-point calibration of the polarographic oxygen sensor.
DatLab converts the raw signal to oxygen concentrations by applying conversion factors determined during a POS calibration comprising an air calibration and a zero calibration. For each gain a different set of calibration values is necessary. Frequently, assumed hardware problems turn out to be a simple calibration problem. This can easily be detected by observing the Raw signal.
Dynamic POS calibration = Calibration of the response time of the POS.


Related O2k-Protocols

» O2k-SOP: MiPNet06.03 POS-Calibration-SOP
» MiPNet21.16 DatLab 7
» MiPNet14.06 InstrumentalO2Background