Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "MiPNet21.17 BloodCellsIsolation"

From Bioblast
(28 intermediate revisions by 8 users not shown)
Line 1: Line 1:
{{OROBOROS header page name}}
{{Publication
{{Publication
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Protocols: Isolation of blood cells for HRR.
|title=[[Image:O2k-Protocols.jpg|right|80px|link=O2k-Protocols|O2k-Protocols]] O2k-Protocols: Isolation of blood cells for HRR.
|info=[[File:PDF.jpg|100px|link=http://wiki.oroboros.at/images/4/47/MiPNet21.17_Blood_cells_isolation.pdf|Bioblast pdf]]
|info=[[File:PDF.jpg|100px|link=http://www.bioblast.at/images/a/af/MiPNet21.17_BloodCellsIsolation.pdf|Bioblast pdf]]Β  Β»[http://www.bioblast.at/index.php/File:MiPNet21.17_BloodCellsIsolation.pdf Versions]
|authors=OROBOROS
|authors=Oroboros
|year=2016
|year=2020-10-22
|journal=Mitochondr Physiol Network
|journal=Mitochondr Physiol Network
|abstract='''Sumbalova Z, Droescher S, Hiller E, Chang S, Garcia L, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17(02):1-15.'''
|abstract=Sumbalova Z, Garcia-Souza LF, Calabria E, Volani C, Gnaiger E (2020) O2k-Protocols: Isolation of peripheral blood mononuclear cells and platelets from human blood for HRR. Mitochondr Physiol Network 21.17(04):1-15.
Β 
{{MiPNet pdf page linking to MitoPedia}}
Respiratory is often performed with isolated mitochondria, tissue homogenate or permeabilized fibres prepared from tissue biopsies. Particularly the latter is invasive and experimentally cumbersome, limiting its applicability. Thus, the alternative use of blood cells, easily obtained by venipuncture, has become increasingly popular in respirometry. Typically whole blood is then separated to obtain either platelets (PLT) or a mixed population of immune cells subsumed as peripheral blood mononuclear cell (PBMCs), both of which have been successfully applied to characterize respiratory phenotypes of human diseases. In this protocol, isolation methods to obtain blood cells for high - resolution respirometry (HRR) are described , important aspects of the isolation procedure are highlighted, and the protocols for the respiratory characterization of platelets and PBMCs are presented.
|mipnetlab=AT_Innsbruck_OROBOROS
|mipnetlab=AT_Innsbruck_OROBOROS
}}
}}
{{Labeling
{{Labeling
|area=Respiration, Instruments;methods
|area=Respiration, Instruments;methods
|organism=Mouse
|organism=Human
|tissues=Blood cells
|tissues=Blood cells, Platelet
|model cell lines=Lymphocyte, Platelet
|preparations=Permeabilized cells, Intact cells
|preparations=Intact cells, Permeabilized cells
|couplingstates=LEAK, ROUTINE, OXPHOS, ET
|couplingstates=LEAK, ROUTINE, OXPHOS, ETS
|pathways=F, N, S, Gp, CIV, NS, ROX
|pathways=F, N, S, Gp, CIV, NS, ROX
|instruments=Oxygraph-2k, O2k-Protocol
|instruments=Oxygraph-2k, O2k-Protocol
|additional=O2k-Demo, Mt-Preparation
|additional=O2k-Demo, Mt-Preparation, MitoEAGLE blood cells reviews,
}}
}}
__TOC__

Revision as of 21:13, 22 October 2020

                



MiPNet21.17 BloodCellsIsolation

Publications in the MiPMap
O2k-Protocols
O2k-Protocols: Isolation of blood cells for HRR.

Β» Bioblast pdf  Β»Versions

Oroboros (2020-10-22) Mitochondr Physiol Network

Abstract: Sumbalova Z, Garcia-Souza LF, Calabria E, Volani C, Gnaiger E (2020) O2k-Protocols: Isolation of peripheral blood mononuclear cells and platelets from human blood for HRR. Mitochondr Physiol Network 21.17(04):1-15.

O2k-technical support and open innovation
Open the pdf document above.
Β» Current O2k-series: NextGen-O2k Series XB and O2k Series J
Β» Current software versions DatLab 8.0: MitoPedia: DatLab


β€’ O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Respiration, Instruments;methods 


Organism: Human  Tissue;cell: Blood cells, Platelet  Preparation: Permeabilized cells, Intact cells 


Coupling state: LEAK, ROUTINE, OXPHOS, ET  Pathway: F, N, S, Gp, CIV, NS, ROX  HRR: Oxygraph-2k, O2k-Protocol 

O2k-Demo, Mt-Preparation, MitoEAGLE blood cells reviews