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MiPNet21.17 BloodCellsIsolation

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Revision as of 11:14, 14 November 2016 by Gnaiger Erich (talk | contribs)
Publications in the MiPMap
O2k-Protocols
O2k-Protocols: Isolation of blood cells for HRR.

Β» Bioblast pdf

OROBOROS (2016-11-13) Mitochondr Physiol Network

Abstract: Sumbalova Z, Hiller E, Chang S, Garcia L, Droescher S, Calabria E, Volani C, Krumschnabel G, Gnaiger E (2016) Isolation of blood cells for HRR. Mitochondr Physiol Network 21.17(02):1-15. Β»Versions

Respiratory assessment of human health and disease can be performed with isolated mitochondria, tissue homogenate or permeabilized fibres prepared from tissue biopsies. The collection of tissue biopsies is invasive, limiting its applicability for large-scale screening. An alternative is the use of blood cells, which can be obtained in a far less invasive sampling procedure. Blood cells obtained by venipuncture are usually separated to obtain platelets (PLT) or a mixed population of immune cells subsumed as peripheral blood mononuclear cell (PBMCs), both of which have been successfully applied to characterize respiratory phenotypes of human diseases. A promising field of innovation is the extended storage or even cryopreservation of blood cells for later use in respirometry. In the present study, isolation methods are described to obtain blood cells for high-resolution respirometry (HRR), methodological details of the isolation procedure are highlighted, and the protocols for the respiratory characterization of platelets and PBMCs are presented.


β€’ O2k-Network Lab: AT_Innsbruck_OROBOROS


Labels: MiParea: Respiration, Instruments;methods 


Organism: Mouse  Tissue;cell: Blood cells, Lymphocyte, Platelet  Preparation: Intact cells, Permeabilized cells 


Coupling state: LEAK, ROUTINE, OXPHOS, ETS"ETS" is not in the list (LEAK, ROUTINE, OXPHOS, ET) of allowed values for the "Coupling states" property.  Pathway: F, N, S, Gp, CIV, NS, ROX  HRR: Oxygraph-2k, O2k-Protocol 

O2k-Demo, Mt-Preparation