MiPNet22.03 IOC119 Innsbruck AT
 Innsbruck AT, 2017 Feb 28-Mar 03. MitoFit Coaching Days - Basic IOC119. |
Oroboros (2017-02-27) Mitochondr Physiol Network
Abstract:
MitoFit Coaching Days - Basic. Innsbruck, Austria; 2017 Feb 28-Mar 03.
• O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit
Programme
Tuesday 28th Topic Room 17:00 - 18:00 Welcome - common aims MiPArt
Wednesday 1st Topic Room 09:00 Mitochondrial and cell research MiPArt 10:00 OroboPOS service and O2k instrumental setup - overview with videoclips MiPArt 10:30 Hands-on: OroboPOS service and O2k instrumental setup MiPArt 12:00 Lunch break 13:00 Instrumental quality control 1: oxygen calibration MiPArt 14:00 Instrumental quality control 2: Instrumental O2 background MiPArt 15:00 Coffee 15:30-18:00 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. ORO-MitoFit Lab
Thursday 2nd Topic Room 09:00 Introduction to SUIT protocols RP1&RP2 MiPArt 10:00 Coffee 10:30 O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with RP1 and RP2. MiPArt 12:00 Lunch break 13:00 Introduction to DatLab analysis MiPArt 15:00 Coffee 15:30-18:00 Individual DatLab analysis + summary ORO-MitoFit Lab
Friday 3rd Topic Room 09:00 SUIT protocols MiPArt 10:30 Coffee 11:00 Q&A session on HRR and OXPHOS analysis: Design of experimental protocols MiPArt 12:00 Lunch break 13:00 O2k-Core and O2k-Multisensor MiPArt 14:00 DatLab analysis ORO-MitoFit Lab 15:00 Coffee 15:30 DatLab analysis ORO-MitoFit Lab 16:15 The Bioblast wiki, O2k-Network and MITOEAGLE - feedback & conclusions MiPArt 17:30 Farewell activity MiPArt
General information
- The MitoFit Coaching Days - Basic provide a fundamental introduction to high-resolution respirometry with the O2k-Core, instrumental assembly and technical service, the DatLab software, oxygen sensor calibration, oxygen flux analysis of respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols.
- Early stage researchers (ESR´s) during their Marie Skłodowska-Curie - TRACT project will be trained at the O2k-Workshop on high-resolution respirometry (HRR).
- » Information on travel and venue: IOC Innsbruck.
Registration
- Fully booked.
- Maximum number of 6 participants.
Tutors
Participants
Participant Institution Anja Bufe AT Innsbruck Oroboros : Oroboros Instruments, Innsbruck (AT) Eugenia Carvalho US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) Matthew Cotter US AR Little Rock Boersheim E: Arkansas Children’s Hospital Research Institute, Little Rock, AR (US) Ivone Leong SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE) Yue Hugh Guan Shanghai (CN) Vasco Sequeira Oliveira NL Amsterdam Wuest RC: VU University Medical Center, Amsterdam (NL) Anna-Maria Wintler SE Stockholm Wikstroem J: Karolinska Institute, Stockholm (SE) Evelyn Zacharewicz NL Maastricht Schrauwen P: Department of Human Biology and Movement Sciences, Maastricht University (NL)
Contact
- Oroboros Instruments
- high-resolution respirometry
- Schöpfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 512 566796
- Fax: +43 512 566796 20
- Mitochondria and cell research
Project support
- Contribution to K-Regio project MitoFit, funded by the Land Tirol within the program K-Regio of Standortagentur Tirol.
IOC recommended reading
O2k-Workshops and MitoFit Coaching Days are listed as MitoGlobal Events.
Labels:
HRR: Oxygraph-2k
ORO, IOC, MitoFit, TRACT, 2017
