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Difference between revisions of "MiPNet25.06 IOC145 Innsbruck AT"

From Bioblast
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| [[File:Profile-icon-9.png|70px|Participant]]
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|Β  [[Pilotti Andrea]]
|Β  [[Pilotti Andrea]]
| '''[[IT Udine Grassi B]]''' -University of Udine, Udine (IT)
| '''[[IT Udine Grassi B]]''' - University of Udine, Udine (IT)


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Revision as of 08:49, 17 December 2019

                



MiPNet25.06 IOC145 Innsbruck AT


Publications in the MiPMap
OROBOROS-solo.png

Innsbruck AT, 2020 Jan 27-29. O2k-Coaching Days - Basic IOC145.


Oroboros (2020-01-27) Mitochondr Physiol Network

Abstract:

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O2k-Coaching Days - Basic. Innsbruck, Austria; 2020 Jan 27-29.


β€’ O2k-Network Lab: AT_Innsbruck_Oroboros, AT_Innsbruck_MitoFit

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General information

The course is fully booked.
O2k-Coaching Days - Basic is a training course which provides a fundamental introduction to high-resolution respirometry (HRR) with the Oroboros O2k. We will give you an overview of the O2k-FluoRespirometer, including real-time analysis with DatLab 7.4 and applications of the Titration-Injection microPump TIP2k. Hands-on sessions range from OroboPOS technical service (instrumental quality control system which is an essential component of HRR) to respirometry experiments with substrate-uncoupler-inhibitor (SUIT) protocols. Also the new Oroboros SUITbrowser will be presented. The SUITbrowser helps you to find the best SUIT protocol for your specific research questions. Many optimized SUIT protocols are available as DL-Protocols and will be shown at the O2k-Coaching Days.


Venue

Β» Oroboros O2k-Laboratory
Schoepfstrasse 18, 6020 Innsbruck
Β» Information on travel and venue: IOC Innsbruck

Registration


Registration closed


Tutors and lecturers


Preliminary programme


Monday 27th

Topic Room
08:15 Welcome - Get-together: Introduction of participants and their research interests Oroboros O2k-Laboratory
08:30 OroboPOS service and O2k instrumental setup - overview with videoclips Oroboros O2k-Laboratory
09:15 Hands-on: OroboPOS service and O2k instrumental setup Oroboros O2k-Laboratory
09:55 DatLab 7.4 overview Oroboros O2k-Laboratory
10:30 Coffee
11:00 Instrumental quality control 1: oxygen calibration Oroboros O2k-Laboratory
11:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: Instrumental quality control 1: oxygen calibration (continuation)

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
13:30 Instrumental quality control 2: Instrumental O2 background – overview with videoclips Oroboros O2k-Laboratory
14:00 Hands-on:Instrumental quality control 2: Instrumental O2 background

DL-Protocol: Instrumental O2 background TiP2k

Oroboros O2k-Laboratory
15:00 Coffee
15:30-17:30 Hands-on: Instrumental quality control 1 and 2. DatLab analysis. Oroboros O2k-Laboratory


Tuesday 28th

Topic Room
08:30 Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
09:15 Introduction to substrate-uncoupler-inhibitor titration (SUIT) protocols – fundamental principles. SUIT reference protocol: RP1&RP2 Oroboros O2k Laboratory
09:45 SUITbrowser: how to find the best SUIT protocol for your research questions. Oroboros O2k Laboratory
10:00 Coffee
10:30 Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).

DL-Protocol: SUIT-001 O2 ce-pce D003.DLP

DL-Protocol: SUIT-002 O2 ce-pce D007.DLP

Oroboros O2k Laboratory
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use (continuation)

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
13:30 DatLab 7.4 analysis: Introduction and new features Oroboros O2k-Laboratory
15:00 Coffee
15:30-17:30 Individual DatLab analysis + summary Oroboros O2k-Laboratory


Wednesday 29th

Topic Room
08:30 MitoFit proficiency test

Hands-on: Instrumental quality control 1: oxygen calibration

DL-Protocol: O2k-cleaning BeforeUse

DL-Protocol: O2 calibration air

Oroboros O2k-Laboratory
10:00 Coffee
10:30 MitoFit proficiency test

Hands-on: O2k-Demo experiment: Respiration of permeabilized cells: measurement of oxygen consumption with the reference protocol RP1 (SUIT-001) and RP2 (SUIT-002).

DL-Protocol: SUIT-001 O2 ce-pce D003.DLP

DL-Protocol: SUIT-002 O2 ce-pce D007.DLP

Oroboros O2k-Laboratory
11:45 Hands-on: O2k-cleaning after use

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
12:00 Lunch break
13:00 Hands-on: O2k-cleaning after use (continuation)

DL-Protocol: O2k-cleaning AfterUse

Oroboros O2k-Laboratory
13:15 DatLab analysis Oroboros O2k-Laboratory
14:00 O2k-Applications - overview Oroboros O2k-Laboratory
14:30 Proficiency test: DatLab analysis summary Oroboros O2k-Laboratory
15:00 Coffee
15:30 The Bioblast wiki, MitoFit Preprint Archives, O2k-Network and MitoEAGLE MiPArt
16:30 Feedback & conclusions MiPArt
17:00 Farewell activity MiPArt

Participants

Participant Institution
Participant Blumkin Moriya IL Tirat Hacarmel Minovia - Minovia Therapeutics LTD, Tirat Hacarmel (IL)
Participant Filippi Celine UK London Filippi C - King's College Hospital, London (UK)
Participant Gurkina Angelina AT Innsbruck Oroboros - Oroboros Instruments, Innsbruck (AT)
Participant Handl Jiri CZ Pardubice Rousar T - University of Pardubice, Pardubice (CZ)
Participant Hofmann Julia AT Innsbruck Gnaiger E - DSL-Oroboros Research Lab, Innsbruck (AT)
Participant Micankova Petra CZ Pardubice Rousar T - University of Pardubice, Pardubice (CZ)
Oefele Marlene Oefele Marlene DE Seewiesen Casagrande S - Max Planck Institute for Ornithology, Seewiesen (DE)
Participant Pilotti Andrea IT Udine Grassi B - University of Udine, Udine (IT)

Contact

Oroboros Instruments GmbH
High-Resolution Respirometry
Schoepfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
E-Mail: instruments@oroboros.at
Mitochondria and Cell Research


IOC recommended reading

Β» IOC recommended reading
Β» MitoPedia: Respiratory states
OXPHOS ROUTINE ETS LEAK ROX


MitoGlobal
O2k-Workshops and O2k-Coaching Days are listed as MitoGlobal Events.


Labels:






ORO, IOC, MitoGlobal, Next, 2020