Difference between revisions of "MiPNet27.05 Schroecken BEC tutorial-Living Communications pmP"

Revision as of 19:24, 10 July 2022

MiPNet27.05 Schroecken BEC tutorial-Living Communications pmP

Schroecken AT, 2022 Sep 30-Oct 02. BEC tutorial-Living Communications: pmF — pre IOC155.

Oroboros (2022-09-30) Mitochondr Physiol Network

Abstract: BEC tutorial-Living Communications. Mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics. Preceding the Oroboros O2k-Workshop on high-resolution respirometry. Schroecken, Austria; 2022.

The mitochondrial membrane potential is an element of the science of bioenergetics, linked to the control of respiratory flux and related mitochondrial functions. A PubMed search on ‘mitochondrial membrane potential’ yields nearly 40 000 results and 3442 for 2021 (search 2022-07-04), with a linear increase during the past 20 years. Chapter 8 on ‘Protonmotive pressure and respiratory control’ of Mitochondrial Pathways (Gnaiger 2020) introduces a novel perspective on Peter Mitchell’s protonmotive force, which incorporates the mitochondrial membrane potential. If you find the reading is tough, you are not alone. Join this BEC tutorial-Living Communications for a fundamental introduction into the relevant concepts of physical chemistry, which differ from misleading chapters in bioenergetics textbooks. A retreat with plenty of informal discussions and group interactions takes you on a journey to visit chemical potential differences versus potential gradients, Gibbs energy versus Gibbs force, quantities of capacity versus intensity, protonmotive force and motive units, flows and forces, and finally protonmotive pressure. This will introduce students (and teachers) to a new understanding of mitochondrial membrane potential and the protonmotive force, connecting the ideal gas equation, osmotic pressure, the Boltzmann constant and gas constant with Fick’s and Einstein’s diffusion equation. If theory gets dry and grey, join for a swim in lake Körbersee, for a Walk&Talk in the colorful alpine environment of the Schröcken-Tannberg region, and a visit to the Alpmuseum ufm Tannberg.

• O2k-Network Lab: AT_Innsbruck_Oroboros

General information

COVID-19

The event will be held in accordance with current COVID regulations. A primary concern must be the safety of our participants and staff, which is why we reserve the right to cancel the event if there are any concerns/restrictions. Refunds will be issued exclusively for registration fees.

Venue

Hotel Körbersee, Schröcken, AT

Information on travel and venue: IOC Schroecken.

» As there is no ATM close to the hotel, we recommend withdrawing money at Bregenz train station. Please make sure to bring some cash in EUROs with you, in case you need it.

The hotel accepts payment by credit card for consumed drinks.

» Please note that the hotel has a limited number of rooms and space is not guaranteed. Therefore, we ask that you to not bring additional guests without confirming with us beforehand.

Program

Figure 8.5. Gibbs energy as a function of advancement of transformation in a closed isothermal system at constant pressure.

BEC tutorial: program for 2022 Sep 30-Oct 02 (Fr to Su)

Preceding the Oroboros O2k-Workshop on high-resolution respirometry, 2022 Oct 03-08 (Mo to Fr).

Peter Mitchell's concept of the protonmotive force pmF is one of the grand unifying theories of biology, on par with Charles Darwin's theory of evolution, Gregor Mendel's rules of inheritance and classical genetics, and the structure of DNA resolved by Francis Crick, James Watson, and Rosalind Franklin. The pmF combines the disciplines of biochemistry (metabolism), cell biology (cell structure), physiology (energy transformation), and physical chemistry (diffusion, electrochemistry), and thermodynamics (chemical potential, Gibbs energy).

Forget all gibberish that you have learned — if not forgotten already — on thermodynamics from bioenergetics and biochemistry textbooks. If you are surprised by this suggestion, take a look at a »specific example.

Registration

This event is taking place immediately before the O2k-Workshop - IOC155.

Download registration form: download pdf

Lecturer

Erich Gnaiger, PhD., Oroboros Instruments - author of
Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis.

Participants

preliminary

Luiza Cardoso, PhD., Oroboros Instruments, Mitochondrial Wizard
Cristiane Cecatto, PhD., Oroboros Instruments, Mitochondrial Phoenix
Erich Gnaiger, PhD., Oroboros Instruments - CEO, Innovation Alchemist
Mateus Grings , PhD., Oroboros Instruments, Mitochondrial Jedi
Elettra Leo, Dr., Oroboros Instruments
Rafael Moreno-Sanchez, Prof., Oroboros Instruments
Sabine Schmitt, PhD., Oroboros Instruments, Mitochondrial Detective

Contact

instruments@oroboros.at

Oroboros Instruments

High-Resolution Respirometry

Schoepfstrasse 18

A-6020 Innsbruck, Austria

Tel: +43 512 566796

Fax: +43 512 566796 20

Mitochondria and Cell Research

Keywords: Force and membrane potential

Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>

Fundamental relationships

» Force

» Affinity

» Flux

» Advancement

» Advancement per volume

» Stoichiometric number

mt-Membrane potential and protonmotive force

» Protonmotive force

» Mitochondrial membrane potential

» Chemical potential

» Faraday constant

» Format

» Uncoupler

O2k-Potentiometry

» O2k-Catalogue: O2k-TPP+ ISE-Module

» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE

» TPP - O2k-Procedures: Tetraphenylphosphonium

» Specifications: MiPNet15.08 TPP electrode

» Poster

» Unspecific binding of TPP+

» TPP+ inhibitory effect

O2k-Fluorometry

» O2k-Catalogue: O2k-FluoRespirometer

» O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual

» Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin

» TMRM - O2k-Procedures: TMRM

O2k-Publications

» O2k-Publications: mt-Membrane potential

» O2k-Publications: Coupling efficiency;uncoupling

BEC-Living Communications are listed as MitoGlobal Events.

Labels:

ORO, IOC, 2022, MitoGlobal, Next

@@ Line 24: / Line 24: @@
 == Venue ==
 [[File:Schroecken Wiki.JPG|right|330px]]
+:::: Hotel Körbersee, Schröcken, AT
 :::: Information on travel and venue: '''[[IOC Schroecken]]'''.
@@ Line 32: / Line 33: @@
 == Program ==
-::::* Preceding the [[MiPNet27.04 IOC155 Schroecken AT |Oroboros O2k-Workshop on high-resolution respirometry]], 2022 Oct 03-08 (Mo to Fr).
+[[File:Gibbs energy advancement.png|right|330px|link=Gnaiger_2020_BEC_MitoPathways#Chapter_8._Protonmotive_pressure_and_respiratory_control |Gibbs energy and advancement||thumb|Figure 8.5. Gibbs energy as a function of advancement of transformation in a closed isothermal system at constant pressure.]]
-::::* '''BEC tutorial''': program for 2022 Sep 30-Oct 02 (Fr to Su) will be provided shortly.
+::::* '''BEC tutorial''': program for 2022 Sep 30-Oct 02 (Fr to Su)
+::::::* Preceding the [[MiPNet27.04 IOC155 Schroecken AT |Oroboros O2k-Workshop on high-resolution respirometry]], 2022 Oct 03-08 (Mo to Fr).
+::::* Peter Mitchell's concept of the protonmotive force ''pmF'' is one of the grand unifying theories of biology, on par with Charles Darwin's theory of evolution, Gregor Mendel's rules of inheritance and classical genetics, and the structure of DNA resolved by Francis Crick, James Watson, and Rosalind Franklin. The ''pmF'' combines the disciplines of biochemistry (metabolism), cell biology (cell structure), physiology (energy transformation), and physical chemistry (diffusion, electrochemistry), and thermodynamics (chemical potential, Gibbs energy).
+::::* Forget all gibberish that you have learned — if not forgotten already — on thermodynamics from bioenergetics and biochemistry textbooks. If you are surprised by this suggestion, take a look at a »[[Force#Thermodynamic_ignorance |specific example]].
@@ Line 59: / Line 65: @@
 File:Erich Gnaiger.jpg|'''[[Gnaiger E |Erich Gnaiger]]''', PhD., Oroboros Instruments - CEO, ''Innovation Alchemist''
 File:Mateus.jpg|'''[[Grings Mateus|Mateus Grings ]]''', PhD., Oroboros Instruments, ''Mitochondrial Jedi''
-File:Profile-icon-9.png|'''[[Leo Elettra]]''', Dr., Oroboros Instruments
+File:Profile-icon-9.png|'''[[Leo Elettra|Elettra Leo]]''', Dr., Oroboros Instruments
-File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', Dr., Oroboros Instruments, ''Mitochondrial Detective''
 File:MorenoR.jpg|'''[[Moreno-Sanchez Rafael|Rafael Moreno-Sanchez]]''', Prof., Oroboros Instruments
+File:SchmittS.jpg|'''[[Schmitt S|Sabine Schmitt]]''', PhD., Oroboros Instruments, ''Mitochondrial Detective''
 |-
 </gallery>