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MiPNet27.05 Schroecken BEC tutorial-Living Communications pmP

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MiPNet27.05 Schroecken BEC tutorial-Living Communications pmP

Publications in the MiPMap
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Schroecken AT, 2022 Sep 30-Oct 02. BEC tutorial-Living Communications: pmF — pre IOC155.


Oroboros (2022-09-30) Mitochondr Physiol Network

Abstract: BEC tutorial-Living Communications. Mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics. Preceding the Oroboros O2k-Workshop on high-resolution respirometry. Schroecken, Austria; 2022.

Gnaiger 2020 BEC MitoPathways

The mitochondrial membrane potential is an element of the science of bioenergetics, linked to the control of respiratory flux and related mitochondrial functions. A PubMed search on ‘mitochondrial membrane potential’ yields nearly 40 000 results and 3442 for 2021 (search 2022-07-04), with a linear increase during the past 20 years. Chapter 8 on ‘Protonmotive pressure and respiratory control’ of Mitochondrial Pathways (Gnaiger 2020) introduces a novel perspective on Peter Mitchell’s protonmotive force, which incorporates the mitochondrial membrane potential. If you find the reading is tough, you are not alone. Join this BEC tutorial-Living Communications for a fundamental introduction into the relevant concepts of physical chemistry, which differ from misleading chapters in bioenergetics textbooks. A retreat with plenty of informal discussions and group interactions takes you on a journey to visit chemical potential differences versus potential gradients, Gibbs energy versus Gibbs force, quantities of capacity versus intensity, protonmotive force and motive units, flows and forces, and finally protonmotive pressure. This will introduce students (and teachers) to a new understanding of mitochondrial membrane potential and the protonmotive force, connecting the ideal gas equation, osmotic pressure, the Boltzmann constant and gas constant with Fick’s and Einstein’s diffusion equation. If theory gets dry and grey, join for a swim in lake Körbersee, for a Walk&Talk in the colorful alpine environment of the Schröcken-Tannberg region, and a visit to the Alpmuseum ufm Tannberg.


O2k-Network Lab: AT_Innsbruck_Oroboros

Vector flux and velocity

General information

COVID-19

The event will be held in accordance with current COVID regulations. A primary concern must be the safety of our participants and staff, which is why we reserve the right to cancel the event if there are any concerns/restrictions. Refunds will be issued exclusively for registration fees.

Venue

Schroecken Wiki.JPG
Information on travel and venue: IOC Schroecken.
» As there is no ATM close to the hotel, we recommend withdrawing money at Bregenz train station. Please make sure to bring some cash in EUROs with you, in case you need it.
The hotel accepts payment by credit card for consumed drinks.
» Please note that the hotel has a limited number of rooms and space is not guaranteed. Therefore, we ask that you do not bring additional guests without confirming with us beforehand.

Program

preliminary


Registration

Part1: BEC tutorial-Living Communications: pmF (Sep 30-Oct 02). Covering mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics
Part2: Oroboros O2k-Workshop - IOC155 (Oct 03-08). Covering: O2k Basic, Quality Control and NextGen-O2k topics
Download registration form: download pdf

Lecturer



Participants

preliminary

Contact

instruments@oroboros.at
Oroboros Instruments
High-Resolution Respirometry
Schoepfstrasse 18
A-6020 Innsbruck, Austria
Tel: +43 512 566796
Fax: +43 512 566796 20
Mitochondria and Cell Research

Recommended reading

Hydrogen ion circuit and coupling in OXPHOS
Gnaiger 2020 BEC MitoPathways
  1. Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 - Chapter 8
  2. Gnaiger E (2021) The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. https://doi.org/10.26124/mitofit:200004.v2
  3. Gnaiger E (2018) The protonmotive force under pressure: an isomorphic analysis. EBEC 2018 Abstract.
  4. Gnaiger E (2017) Protonmotive force and chemiosmotic pressure: a generalization of non-ohmic flux control of the proton leak. MiP2017 Abstract.
  5. Komlódi T, Tretter L (2022) The protonmotive force – not merely membrane potential. https://doi.org/10.26124/mitofit:2022-0012
  6. Krumschnabel G, Eigentler A, Fasching M, Gnaiger E (2014) Use of safranin for the assessment of mitochondrial membrane potential by high-resolution respirometry and fluorometry. https://doi.org/10.1016/B978-0-12-416618-9.00009-1

Keywords: Force and membrane potential


Questions.jpg


Click to expand or collaps
Bioblast links: Force and membrane potential - >>>>>>> - Click on [Expand] or [Collapse] - >>>>>>>
Fundamental relationships
» Force
» Affinity
» Flux
» Advancement
» Advancement per volume
» Stoichiometric number
mt-Membrane potential and protonmotive force
» Protonmotive force
» Mitochondrial membrane potential
» Chemical potential
» Faraday constant
» Format
» Uncoupler
O2k-Potentiometry
» O2k-Catalogue: O2k-TPP+ ISE-Module
» O2k-Manual: MiPNet15.03 O2k-MultiSensor-ISE
» TPP - O2k-Procedures: Tetraphenylphosphonium
» Specifications: MiPNet15.08 TPP electrode
» Poster
» Unspecific binding of TPP+
» TPP+ inhibitory effect
O2k-Fluorometry
» O2k-Catalogue: O2k-FluoRespirometer
» O2k-Manual: MiPNet22.11 O2k-FluoRespirometer manual
» Safranin - O2k-Procedures: MiPNet20.13 Safranin mt-membranepotential / Safranin
» TMRM - O2k-Procedures: TMRM
O2k-Publications
» O2k-Publications: mt-Membrane potential
» O2k-Publications: Coupling efficiency;uncoupling



MitoGlobal
BEC-Living Communications are listed as MitoGlobal Events.


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