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Difference between revisions of "MiPNet27.06 Prague BEC tutorial-Living Communications pmF"

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  Last update: 2022-09-09
  Last update: 2022-09-16
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== Program ==
== Program ==
::::* Blocks of 10 + 5 min comments, questions, discussion


:::: {| class="wikitable"
:::: {| class="wikitable"
|+ '''Thursday, Sep 22'''
|+ '''Thursday, Sep 22''' — [[Protonmotive force]] ''pmF'' = Δ<sub>m</sub>''F''<sub>H<sup>+</sup></sub> and '''protonmotive pressure Δ<sub>m</sub>''Π''<sub>H<sup>+</sup></sub>'''
|-
! Time !! Section !! Topic !! a !! b !! c !! d
|-
| 10:00-10:10 || 8.1.-8.3. || '''Overview''' || ''pmF'' equation || Mitchell's four modules || three introductory topics || <br>
|-
| 10:15-10:25 || 8.4. || '''1. Four protonmotive theorems''' || diffusive pressure gradient || pressure difference and potential difference || diffusive and electric pressure difference || concave to convex flow/force <br>
|-
| 10:30-10:40 || 8.1. || '''2. [[Vector |Vectorial]] and [[scalar]] quantities''' || [[amount]] and [[charge]] format || diffusion: gradients and [[Discontinuous system |compartments]] || [[Stoichiometric number |stoichiometry]] and [[advancement]] || <br>
|-
| 10:45-10:55 || 8.2. || '''3. From thermodynamics to [[ergodynamics]]''' || [[chemical potential]] || metabolic [[force]] || Gibbs energy - [[exergy]] || [[Elementary entity |elementary quantities]] and [[motive unit]]s <br>
|-
| 11:00-11:10 || 8.3. || '''4. Protonmotive force and motive units''' || ''pmF'' [[format]]s || conversion between formats || measurement of the ''pmF'' || <br>
|-
| 11:15-11:45 || || ''Coffee/tea - Chat''
|-
| 11:45-11:55 || 8.4.1. || '''1. Protonmotive pressure ''pmP'' linearity''' || diffusion gradients and [[Einstein 1905 Ann Physik 549 |Einstein's diffusion equation]] || [[Fick 1855 Pogg Ann |Fick's law]] || [[Pressure |pressure-force confusion]] || thermodynamics of irreversible processes <br>
|-
|-
! Time !! Section !! Contents
| 12:00-12:10 || 8.4.2. || '''2. Compartments: diffusion and osmotic pressure''' || concave flux/force relation || free activity || infinite forces without explosion || <br>
|-
|-
| 10:00-11:30 || Introduction || Protonmotive force ''pmF'' = Δ<sub>m</sub>''F''<sub>H<sup>+</sup></sub> <br>
| 12:15-12:25 || 8.4.3. || '''3. [[Hydrogen ion]]s and counterions''' || if the force is in ∆pH || electroneutral exchange of counterions || equilibrium H<sup>+</sup> and counterion distribution || concave flow (pressure)/force relation <br>
# Mitchell's theorems on four integrated coupling modules <br>
# Is the protonmotive force — units [mV] or [kJ/mol] — a force? <br>
# Is the "Gibbs ''energy''" [kJ/mol] a force? <br>
# Why did Peter Mitchell use the symbol Δp?
|-
|-
| 11:30-12:00 || Coffee/tea || Chat
| 12:30-12:45 || 8.4.4. || '''4. [[Mitochondrial matrix |Matrix]] volume fraction and flux-pressure linearity''' || anodic volume fraction || closed to open anodic system || from [[Intensive quantity |intensity]] to capacity || non-Ohmic [[LEAK respiration |proton leak]] explained by first principles <br>
|-
|-
| 12:00-13:00 || Extension || Four protonmotive theorems on protonmotive ''pressure'' Δ<sub>m</sub>''Π''<sub>H<sup>+</sup></sub> <br>
| 12:45-13:00 || || '''General discussion''' ''with a glass of wine - a taste of [[Gentle Science]]''
# Diffusion gradients <br>
# Compartments: diffusion and osmotic pressure <br>
# Hydrogen ions and counterions <br>
# Matrix volume fraction and flux-pressure linearity
|}
|}


::::» [[BEC tutorial-Living Communications: pmF to pmP |Programme: BEC tutorial-Living Communications: ''pmF'' to ''pmP'']]
::::» [[BEC tutorial-Living Communications: pmF to pmP |'''Programme: BEC tutorial-Living Communications: ''pmF'' to ''pmP''''']]
    
    
== Lecturer and participants ==
== Lecturer and participants ==

Revision as of 05:34, 16 September 2022


Bioenergetics Communications        
Gnaiger 2020 BEC MitoPathways
       
Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1.
        MitoPedia: BEC         MitoPedia: Gentle Science         MitoFit Preprints         DOI Data Center
Publications in the MiPMap
BEC-logo.png

Prague CZ, 2022 Sep 22. BEC tutorial-Living Communications: pmF — pre EMC2022 Prague.


MiPsociety (2022-09-22) Mitochondr Physiol Network

Abstract: BEC tutorial-Living Communications. Mitochondrial membrane potential and Peter Mitchell’s protonmotive force: elements of the science of bioenergetics. Preceding the EMC 2022 49th European Muscle Conference, Prague, Czech Republic.

Gnaiger 2020 BEC MitoPathways

The mitochondrial membrane potential is an element of the science of bioenergetics, linked to the control of respiratory flux and related mitochondrial functions. A PubMed search on ‘mitochondrial membrane potential’ yields nearly 40 000 results and 3442 for 2021 (search 2022-07-04), with a linear increase during the past 20 years. Chapter 8 on ‘Protonmotive pressure and respiratory control’ of Mitochondrial Pathways (Gnaiger 2020) introduces a novel perspective on Peter Mitchell’s protonmotive force, which incorporates the mitochondrial membrane potential. If you find the reading is tough, you are not alone. Join this BEC tutorial-Living Communications for an introduction into the relevant concepts of physical chemistry, which differ from misleading chapters in bioenergetics textbooks on potential gradients, Gibbs energy, protonmotive flow and force, and finally protonmotive pressure. This will introduce students (and teachers) to a new understanding of mitochondrial membrane potential and the protonmotive force, connecting the ideal gas equation, osmotic pressure, the Boltzmann constant and gas constant with Fick’s and Einstein’s diffusion equation. If theory gets tough, join for a follow-up retreat.


O2k-Network Lab: AT_Innsbruck_Oroboros, CZ Prague Houstek J

Figure 1.1. Coupling in oxidative phosphorylation is mediated by the protonmotive force pmF.



Last update: 2022-09-16


MiPsociety
Dr. Zdenek Drahota received from his team in Prague an Oroboros cake at his 80th birthday

This MiPevent is dedicated to Dr. Zdenek Drahota — one of the greatest mitochondrial physiologists of the Czech Republic — at his 90th birthday:

In collaboration with the Mitochondrial Physiology Society - see MiP2017


Venue and local organizers

Laboratory of Bioenergetics
Institute of Physiology CAS
Videnska 1083
142 20 Prague 4
Czech Republic


Program

  • Blocks of 10 + 5 min comments, questions, discussion
Thursday, Sep 22Protonmotive force pmF = ΔmFH+ and protonmotive pressure ΔmΠH+
Time Section Topic a b c d
10:00-10:10 8.1.-8.3. Overview pmF equation Mitchell's four modules three introductory topics
10:15-10:25 8.4. 1. Four protonmotive theorems diffusive pressure gradient pressure difference and potential difference diffusive and electric pressure difference concave to convex flow/force
10:30-10:40 8.1. 2. Vectorial and scalar quantities amount and charge format diffusion: gradients and compartments stoichiometry and advancement
10:45-10:55 8.2. 3. From thermodynamics to ergodynamics chemical potential metabolic force Gibbs energy - exergy elementary quantities and motive units
11:00-11:10 8.3. 4. Protonmotive force and motive units pmF formats conversion between formats measurement of the pmF
11:15-11:45 Coffee/tea - Chat
11:45-11:55 8.4.1. 1. Protonmotive pressure pmP linearity diffusion gradients and Einstein's diffusion equation Fick's law pressure-force confusion thermodynamics of irreversible processes
12:00-12:10 8.4.2. 2. Compartments: diffusion and osmotic pressure concave flux/force relation free activity infinite forces without explosion
12:15-12:25 8.4.3. 3. Hydrogen ions and counterions if the force is in ∆pH electroneutral exchange of counterions equilibrium H+ and counterion distribution concave flow (pressure)/force relation
12:30-12:45 8.4.4. 4. Matrix volume fraction and flux-pressure linearity anodic volume fraction closed to open anodic system from intensity to capacity non-Ohmic proton leak explained by first principles
12:45-13:00 General discussion with a glass of wine - a taste of Gentle Science
» Programme: BEC tutorial-Living Communications: pmF to pmP

Lecturer and participants

Participants

Restricted number of participants: countmax = (29 + 1) x

Registration and general information

  • Informal, no registration fee - send Email to: instruments@oroboros.at
  • Limited number of participants
  • Provide your name and affiliation (if you wish for the website)
  • Provide a foto (if you wish for the website)

COVID-19

The event will be held in accordance with current COVID regulations. A primary concern must be the safety of our participants and staff, which is why we reserve the right to cancel the event if there are any concerns/restrictions.


Recommended reading

Hydrogen ion circuit and coupling in OXPHOS
Gnaiger 2020 BEC MitoPathways
  1. Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. https://doi.org/10.1016/j.bbabio.2011.09.018
  2. Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. https://doi.org/10.26124/bec:2020-0002 - Chapter 8
  3. Gnaiger E (2021) The elementary unit — canonical reviewer's comments on: Bureau International des Poids et Mesures (2019) The International System of Units (SI) 9th ed. https://doi.org/10.26124/mitofit:200004.v2
MitoPedia



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BEC tutorials are listed as MitoGlobal Events.


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