From Bioblast
MiPNet28.12 IOC167 Schroecken AT
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Oroboros (2024-09-30) Mitochondr Physiol Network
Abstract: Oroboros O2k-Workshop on high-resolution respirometry. Schroecken, Austria (2024 Sep 30 - Oct 05).
• O2k-Network Lab: AT_Innsbruck_Oroboros
Feedback
- Please help us to improve the O2k-Workshops by taking a few minutes to answer the following questions: IOC167 Feedback
- This workshop is great for people who want to get started with O2k as well as for people like me who are already using O2k but are not sure if they are doing everything right. I left the workshop feeling much more confident about using O2k and analyzing data from O2k. For me, the balance between theory and practice was just right, the location of the workshop was amazing and the team was awesome. Erich, Eleonora, Sabine and Alba, thank you so much, not only for the presentations and demonstrations, but also for being so friendly, approachable and patient with us. I highly recommend this workshop to anyone looking to improve their understanding and use of O2k. - Dolinar Klemen
- I liked the community including staff of Oroboros instruments and other participants. The hands-on training sessions were the most useful and the theoretical lectures were interesting and inspiring. - Umbrasas Danielius
| Question | Rating (average) 1-5 |
|---|---|
| In how far were your interests covered in the workshop? Please rate from 1 (not covered) to 5 (all interests covered). | 4.6 |
| Balance between practical “hands-on” work and “theoretical” explanations: Please rate from 1 (too much “hands-on”) to 5 (too much theory). | 3.7 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). a. in terms of content: | 4.3 |
| Presentation of lectures: Please rate from 1 (not understandable) to 5 (very understandable). b. in terms of language: | 4.7 |
| Level of tutoring during the “hands-on” work: Please rate from 1 (not enough tutoring, I felt left alone) to 5 (too much tutoring, I would have liked to work more independently). | 3.3 |
| Quality of tutoring: Please rate from 1 (poor) to 5 (excellent). | 5.0 |
| Intensity of the O2k-Course: Please rate from 1 (I felt overwhelmed) to 5 (I would have liked to do and hear more). | 2.9 |
| Your overall rating of the course? Please rate from 1 (poor) to 5 (excellent). | 5.0 |
General information
- This training course on the Oroboros O2k and NextGen-O2k provides an introduction to DatLab 8 and applications of the TIP2k. Demos and hands-on experiments illustrate the principle and show the unique advantages and limitations of simultaneous monitoring of oxygen concentration and mitochondrial respiration. The O2k-Fluo Smart-Module is presented with hands-on calibrations for Amplex UltraRed (hydrogen peroxide, ROS) or safranin (mitochondrial membrane potential). Many optimized SUIT protocols are available as DL-Protocols. The SUITbrowser helps you find the best SUIT protocol for your specific research questions. Furthermore, this workshop covers interpretation as well as calibration and instrumental service.
Delays
- We would like to advise you to make sure to book flights that will allow you to arrive in Bregenz in time for the scheduled bus pickup.
- In the case of delay, please call Oroboros Instruments Mobile: +43 677 64 79 29 17 or Oroboros Office: +43 512 566 796 (open until 5:30 pm) as soon as you know you will arrive late.
- If you miss the scheduled transportation you will have to take a cab (Suggestion: Ritter Transport, Phone number: +43 664 34 11 540; or any local cab) or the public transportation to Schroecken.
- If you decide to take the public transportation, these are the bus routes you may take. You must change bus at least one time and exit at Schröcken Dorf:
- Please be aware that you will be responsible for paying for any additional transportation costs (about € 150 for a cab/ travel time 1 h; about € 11 for the public transportation / travel time 1.5 h - 2 h). Please check with your airline to see if there are any reimbursements available in the event that you miss the organized transport and have to arrange and pay for a cab or public transportation.
Program
- Program found here:
- Program found here:
Tutors and lecturers
Eleonora Baglivo, MSc, Oroboros Instruments, Biomedical Pixie
Erich Gnaiger, PhD, Oroboros Instruments - CEO, Innovation Alchemist
Sabine Schmitt, Dr., Oroboros Instruments, Mitochondrial Detective
Alba Timon-Gomez, PhD, Oroboros Instruments, Mitochondrial Mage
Klemen Dolinar, Univ Dipl Biochem, Invited Guest Tutor, University of Ljubljana - SI Ljubljana Pirkmajer S
Organizer and participant
Carolina Gnaiger, MSc, Oroboros Instruments, Project Management Elf'
Participants
| Participant | Institution | ||
|---|---|---|---|
| 1. | 
|
Umbrasas Danielius | LT Kaunas Baniene R - Lithuanian University of Health Sciences, LT |
| 2. | 
|
Katakam Prasad | US LA New Orleans Katakam PV - Tulane University School of Medicine, US |
| 3. | 
|
Millet Caroline | UK Glasgow Metcalfe NB - University of Glasgow, UK |
| 4. |
|
Choi Ran Hee | US UT Salt Lake City Funai K - University of Utah, US |
| 5. |
|
Radovic Marko | University Medical Center Groningen, NL |
| 6. | 
|
Lira Chavez Fernanda Montserrat | University Medical Center Groningen, NL |
| 7. | 
|
Pedrosa Mafalda | PT Porto Magalhaes J - University of Porto, PT |
| 8. |
|
Starke Ina | University of Veterinary Medicine, DE |
| 9. |
|
O'Kelly Benjamin | Eli Lilly and Company, US |
| 10. | 
|
Buck Amy | ZA_Cape Town_Maarman GJ - Stellenbosch University, ZA |
| 11. |
|
Thorne Anneke | Northwestern University, US |
| 12. | 
|
Nielsen Grace | University of Nebraska, US |
| 13. |
|
Ulbrich Russell | ScientiaLux LLC, US |
| 14. | 
|
Marmisolle Ines | UY Montevideo Radi R - Universidad de la Republica, UY |
| 15. | 
|
Verduci Marzia | University of Veterinary Medicine, AT |
O2k perspectives - abstracts
- Abstracts for the O2k perspectives evenings provide participants an opportunity to present and discuss their work, including projects in progress, methodological details and conceptual perspectives.
- » Abstract guidelines
| Has title | |
|---|---|
| Buck 2024 Abstract IOC167 | Buck AC, Dube A, Maarman G, Bardien S (2024) Curcumin-encapsulated nanoparticles to target mitochondrial dysfunction in a cellular model of Parkinson’s disease. Mitochondr Physiol Network 28.12. |
| Umbrasas 2024 Abstract IOC167 | Umbrasas D, Pampuščenko K, Gružaitė J, Morkūnienė R, Smirnovas V, Borutaitė V (2024) Extracellular alpha-synuclein and S100A9 species cause a shift in microglial energy metabolism. Mitochondr Physiol Network 28.12. |
Impressions from IOC167
IOC167:
Walk to Koerbersee
Walk to Koerbersee
IOC167 Arrival with welcome Schnaps
DEMO living cells by Erich
Group 3 Hands-on Oxygen calibration
Group 5 Hands-on Oxygen calibration
Handling the TIP2k
Hands-on IOC167
Hands-on Chamber assembly
Hands-on Volume calibration
Hands-on OroboPOS service
Group photo IOC167
Group photo IOC167
O2k-Workshop quiz requires concentration...
... and whispering.
O2k-Workshop quiz can be scary
People get serious if they do not win
but still have a lot of fun
and some more fun
but there can only be one winning team at the O2k-Workshop Quiz
Walks & Talks:
Walk and talk
Group at the Koerbersee
Game at the Alpmuseum
Game at the Alpmuseum
Visit at the Alpmuseum
Visit at the Alpmuseum
Oroboros Team at the Koerbersee
Venue
- Hotel Körbersee, Schroecken, AT
- Information on travel and venue: IOC Schroecken.
- » As there is no ATM close to the hotel, we recommend withdrawing money at Bregenz train station. Please make sure to bring some cash in EURO with you, in case you need it.
- The hotel accepts payment by credit card for consumed drinks.
- » As there is no ATM close to the hotel, we recommend withdrawing money at Bregenz train station. Please make sure to bring some cash in EURO with you, in case you need it.
- » Please note that the hotel has a limited number of rooms and space is not guaranteed. Rooms are allocated on the basis of first come, first serve. Further, we ask that you to confirm with us in advance if you intend to bring accompanying guests with you.
Contact
- Oroboros Instruments
- High-Resolution Respirometry
- Schoepfstrasse 18
- A-6020 Innsbruck, Austria
- Tel: +43 677 647 929 17
- Fax: +43 512 566796 20
- Mitochondria and Cell Research
IOC recommended reading
- The Must-Read for the O2k-Workshop:
- Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. - »Bioblast link« - A new edition with updated terminology and more ..
- » Chapters 4-5: NADH-linked pathways through Complex CI and S-pathway through Complex CII
- » Chapter 6: Convergent electron transfer at the Q-junction
- Further reading:
- » IOC recommended reading
- » MitoPedia: Respiratory states
O2k-Workshops are listed as MitoGlobal Events.
Labels:
ORO, IOC, MitoFit, 2024, MitoGlobal, NextGen-O2k, O2k-Network Award Previous
