Difference between revisions of "MiR05"
From Bioblast
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|info=[[MiR05-Kit]], [[Gnaiger 2000 Life in the Cold]] | |info=[[MiR05-Kit]], [[Gnaiger 2000 Life in the Cold]] | ||
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== Comparison of respiration media == | |||
:::# Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - [[Gnaiger 2000 Life in the Cold |»Bioblast link«]] - The original article describing MiR05. | |||
::::* Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]] - "''when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers''". | |||
== MiR05-Kit in HRR == | |||
::::* For preparation of MiR05, see [[MiR05-Kit]]. | |||
::::* The '''[[oxygen solubility factor]]''' of MiR05 is '''0.92'''. | |||
{{MitoPedia topics | {{MitoPedia topics | ||
|mitopedia topic=Medium | |mitopedia topic=Medium | ||
}} | }} | ||
Revision as of 16:21, 28 April 2019
Description
Mitochondrial respiration medium, MiR05, developed for oxygraph incubations of mitochondrial preparations. MiR06 = MiR05 + catalase. MiR05Cr = MiR05 + creatine.
Abbreviation: MiR05
Reference: MiR05-Kit, Gnaiger 2000 Life in the Cold
Comparison of respiration media
- Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - »Bioblast link« - The original article describing MiR05.
- Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - »Bioblast link« - "when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers".
MiR05-Kit in HRR
- For preparation of MiR05, see MiR05-Kit.
- The oxygen solubility factor of MiR05 is 0.92.
MitoPedia topics:
Media for respirometry