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Difference between revisions of "MiR05"

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{{MitoPedia
{{MitoPedia
|abbr=MiR05
|abbr=MiR05
|description='''Mitochondrial respiration medium, MiR05''', developed for oxygraph incubations of [[mitochondrial preparations]].  [[MiR06]] = MiR05 + catalase.
|description='''Mitochondrial respiration medium, MiR05''', developed for oxygraph incubations of [[mitochondrial preparations]]. Respiration of [[living cells]] may be assessed in MiR05 by adding pyruvate (P) as an external source.  [[MiR06]] = MiR05 + catalase.
[[MiR05Cr]] = [[MiR05]] + creatine.
[[MiR05Cr]] = [[MiR05]] + creatine.
|info=[[MiPNet14.13 Medium-MiR06]]; [[Gnaiger 2000 Life in the Cold]]
|info=[https://www.oroboros.at/index.php/product/mir05-kit/ MiR05-Kit], [[Gnaiger 2000 Life in the Cold]], [[MiPNet22.10 MiR05-Kit]]
|type=Chemicals
}}
{{MitoPedia methods
|mitopedia method=Respirometry
|type=Chemicals
}}
}}
__TOC__
== Comparison of respiration media ==
:::# Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - [[Gnaiger 2000 Life in the Cold |»Bioblast link«]] - The original article describing MiR05 in comparison with the composition of mitochondrial and organ preservation solutions.
:::# Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - [[Krumschnabel_2015_Methods Mol Biol |»Bioblast link«]]
:::# Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - [[Wollenman 2017 PLOS ONE |»Bioblast link«]] - "''when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers''".
:::# Komlódi T, Sobotka O, Krumschnabel G, Bezuidenhoutz N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. - [[Komlodi_2018_Methods Mol Biol |»Bioblast link«]]
:::# Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004
:::# Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - [[Doerrier 2018 Methods Mol Biol |»Bioblast link«]]
:::# Krycer JR, Fisher-Wellman KH, Fazakerley DJ, Muoio DM, James DE (2017) Bicarbonate alters cellular responses in respiration assays. Biochem Biophys Res Commun 489:399-403. - [[Krycer 2017 Biochem Biophys Res Commun |»Bioblast link«]]
== MiR05-Kit in HRR ==
::::* For preparation of MiR05, see [[MiPNet22.10 MiR05-Kit]].
::::* The '''[[oxygen solubility factor]]''' of MiR05 is '''0.92'''.
{{MitoPedia topics
{{MitoPedia topics
|mitopedia topic=Medium
|mitopedia topic=Medium
|type=Chemicals
}}
}}
The '''oxygen solubility factor''' of MiR05 is '''0.92'''.

Revision as of 07:43, 1 September 2022


high-resolution terminology - matching measurements at high-resolution


MiR05

Description

Mitochondrial respiration medium, MiR05, developed for oxygraph incubations of mitochondrial preparations. Respiration of living cells may be assessed in MiR05 by adding pyruvate (P) as an external source. MiR06 = MiR05 + catalase. MiR05Cr = MiR05 + creatine.

Abbreviation: MiR05

Reference: MiR05-Kit, Gnaiger 2000 Life in the Cold, MiPNet22.10 MiR05-Kit

Comparison of respiration media

  1. Gnaiger E, Kuznetsov AV, Schneeberger S, Seiler R, Brandacher G, Steurer W, Margreiter R (2000) Mitochondria in the cold. In: Life in the Cold (Heldmaier G, Klingenspor M, eds) Springer, Heidelberg, Berlin, New York:431-42. - »Bioblast link« - The original article describing MiR05 in comparison with the composition of mitochondrial and organ preservation solutions.
  2. Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - »Bioblast link«
  3. Wollenman LC, Vander Ploeg MR, Miller ML, Zhang Y, Bazil JN (2017) The effect of respiration buffer composition on mitochondrial metabolism and function. PLOS ONE 12:e0187523. - »Bioblast link« - "when the goal is to maximize ADP-stimulated respiration, buffers containing K-lactobionate or K-gluconate are superior choices compared to the standard KCl-based buffers".
  4. Komlódi T, Sobotka O, Krumschnabel G, Bezuidenhoutz N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55. - »Bioblast link«
  5. Komlódi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. https://doi.org/10.26124/bec:2021-0004
  6. Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, Mészáros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. - »Bioblast link«
  7. Krycer JR, Fisher-Wellman KH, Fazakerley DJ, Muoio DM, James DE (2017) Bicarbonate alters cellular responses in respiration assays. Biochem Biophys Res Commun 489:399-403. - »Bioblast link«

MiR05-Kit in HRR


MitoPedia topics: Media for respirometry