Difference between revisions of "MiR06Cr"
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{{MitoPedia | {{MitoPedia | ||
|abbr=MiR06Cr | |abbr=MiR06Cr | ||
|description=Mitochondrial respiration medium, '''MiR06Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized muscle | |description=Mitochondrial respiration medium, '''MiR06Cr''', developed for oxygraph incubations of mitochondrial preparations - ''[[permeabilized muscle fibers]]''. | ||
MiR06Cr = [[MiR06]] + 20 mM [[Creatine|creatine]]. | MiR06Cr = [[MiR06]] + 20 mM [[Creatine|creatine]]. | ||
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'''Rationale:''' as for [[MiR06]] the presence of catalase is protective, allows for reoxygenations with H<sub>2</sub>O<sub>2</sub> injections, but prevents simultaneous measurement of H<sub>2</sub>O<sub>2</sub> production, and less ADP needed in permeabilized muscle | '''Rationale:''' as for [[MiR06]] the presence of catalase is protective, allows for reoxygenations with H<sub>2</sub>O<sub>2</sub> injections, but prevents simultaneous measurement of H<sub>2</sub>O<sub>2</sub> production, and less ADP needed in permeabilized muscle fibers. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect. | ||
Latest revision as of 18:45, 15 May 2019
- high-resolution terminology - matching measurements at high-resolution
MiR06Cr
Description
Mitochondrial respiration medium, MiR06Cr, developed for oxygraph incubations of mitochondrial preparations - permeabilized muscle fibers.
MiR06Cr = MiR06 + 20 mM creatine.
Abbreviation: MiR06Cr
Reference: MiPNet14.13
MitoPedia topics: Media for respirometry
- For preparation of MiR06Cr and application in HRR, see MiPNet14.13 Medium-MiR06.
- The oxygen solubility factor of MiR06Cr is the same as for MiR05 and MiR05Cr = 0.92.
Rationale: as for MiR06 the presence of catalase is protective, allows for reoxygenations with H2O2 injections, but prevents simultaneous measurement of H2O2 production, and less ADP needed in permeabilized muscle fibers. This is of advantage when using the TPP electrode, because ADP has a high chemical side effect.
