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Difference between revisions of "MitoKit-CII"

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Revision as of 13:00, 25 March 2019

                



MitoKit-CII

O2k-Catalogue

Description Cell permeable compound - MitoKit-CII, 2 vials; 1 vial of NV118 (25 mg) + 1 vial of NV161 (10 mg)
Product ID 60200-01
Type O2k
Link MitoKit-CII MitoPedia
Image

Compounds


The cell permeable compound MitoKit-CII is the first generation of NeuroVive Pharmaceutical AB's succinate/malonate prodrugs and was developed to stimulate and inhibit Complex II of the respiratory electron transfer system in intact cells, and will expand the toolbox for evaluating the role of mitochondria in disease.


Compounds of MitoKit-CII

The MitoKit-CII is composed of equal quantities of the following Compounds:
  • Cell permeable compound NV118
Description: Succinate prodrug: C10H14O8, Molecular weight 262,2g.mol-1
Aliquots per 25 mg


  • Cell permeable compound NV161
Description: Malonate prodrug: C9H12O8, Molecular weight 248,2g.mol-1
Aliquots per 10 mg


  • Reference:
Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial complex I deficiency. Nat Commun 7:12317. »Bioblast link« - Original publication introducing Mito-CII.


Preparation

  • NV118: Dissolved in DMSO for 1M concentration (MW= 262.2 g/mol: 100 mM stock solution) and stored in aliquots at -20.
  • NV161: Dissolved in DMSO for 1M concentration (MW = 248.2 g/mol: 10 mM stock solution) and stored in aliquots at -20.


Expiry date of MitoKit-CII compounds (according to NeuroVive)

Before preparation:

  • 3 years in -80° C
  • 1 year in -20° C


Prepared:

  • In DMSO solution
  • at room temperature: 24 hours.
  • at -20° C: for weeks


CAS-Numbers:

  • NV-118: 1592635-33-0
  • NV-161: 1432752-62-9


Labels: MiParea: Instruments;methods 





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