Difference between revisions of "MitoKit-CII"
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The | The MitoKit-CII consists of the first generation of NeuroVive Pharmaceutical AB's cell permeable succinate and malonate prodrugs. It was developed to stimulate (succinate) or inhibit (malonate) the Complex II of the respiratory electron transfer system in intact cells, and will expand the toolbox for evaluating the role of mitochondria in disease. | ||
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::::* '''Cell permeable compound [[NV118]]''' | ::::* '''Cell permeable compound [[NV118]]''' | ||
::::: Description: Succinate prodrug: C<sub>10</sub>H<sub>14</sub>O<sub>8</sub>, Molecular weight 262,2g.mol<sup>-1</sup> | ::::: Description: Succinate prodrug: C<sub>10</sub>H<sub>14</sub>O<sub>8</sub>, Molecular weight 262,2g.mol<sup>-1</sup> | ||
::::: Aliquots | ::::: Aliquots of 25 mg | ||
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::::: Description: Malonate prodrug: C<sub>9</sub>H<sub>12</sub>O<sub>8</sub>, Molecular weight 248,2g.mol<sup>-1</sup> | ::::: Description: Malonate prodrug: C<sub>9</sub>H<sub>12</sub>O<sub>8</sub>, Molecular weight 248,2g.mol<sup>-1</sup> | ||
::::: Aliquots | ::::: Aliquots of 10 mg | ||
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=== Preparation === | === Preparation === | ||
:::: | :::: Both compounds are dissolved in DMSO for 1M concentration stock solution and stored in aliquots at -20. | ||
::::* NV161 | We recommend the following dilutions for reaching the desired concentration in the experiments: | ||
::::* NV118: 100 mM stock solution | |||
::::* NV161: 10 mM stock solution | |||
Revision as of 15:50, 25 March 2019
MitoKit-CII
Description | Cell permeable compound - MitoKit-CII, 2 vials; 1 vial of NV118 (25 mg) + 1 vial of NV161 (10 mg) |
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Product ID | 60200-01 |
Type | O2k |
Link | MitoKit-CII MitoPedia |
Image |
Compounds
The MitoKit-CII consists of the first generation of NeuroVive Pharmaceutical AB's cell permeable succinate and malonate prodrugs. It was developed to stimulate (succinate) or inhibit (malonate) the Complex II of the respiratory electron transfer system in intact cells, and will expand the toolbox for evaluating the role of mitochondria in disease.
Compounds of MitoKit-CII
- The MitoKit-CII is composed of equal quantities of the following Compounds:
- Cell permeable compound NV118
- Description: Succinate prodrug: C10H14O8, Molecular weight 262,2g.mol-1
- Aliquots of 25 mg
- Cell permeable compound NV161
- Description: Malonate prodrug: C9H12O8, Molecular weight 248,2g.mol-1
- Aliquots of 10 mg
- Reference:
- Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial complex I deficiency. Nat Commun 7:12317. »Bioblast link« - Original publication introducing Mito-CII.
Preparation
- Both compounds are dissolved in DMSO for 1M concentration stock solution and stored in aliquots at -20.
We recommend the following dilutions for reaching the desired concentration in the experiments:
- NV118: 100 mM stock solution
- NV161: 10 mM stock solution
Expiry date of MitoKit-CII compounds (according to NeuroVive)
Before preparation:
- 3 years in -80° C
- 1 year in -20° C
Prepared:
- In DMSO solution
- at room temperature: 24 hours.
- at -20° C: for weeks
CAS-Numbers:
- NV-118: 1592635-33-0
- NV-161: 1432752-62-9
Labels: MiParea: Instruments;methods
HRR: O2k-Protocol
O2k-chemicals and media