Difference between revisions of "MitoKit-CII"
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== Compounds of MitoKit-CII == | == Compounds of MitoKit-CII == | ||
:::: The MitoKit-CII is composed | :::: The MitoKit-CII is composed of the following compounds: | ||
::::* '''Cell permeable compound [[NV118]]''' | ::::* '''Cell permeable compound [[NV118/MitoKit-CII]]''' | ||
::::: Description: Succinate prodrug: C<sub>10</sub>H<sub>14</sub>O<sub>8</sub>, Molecular weight 262,2g.mol<sup>-1</sup> | ::::: Description: Succinate prodrug: C<sub>10</sub>H<sub>14</sub>O<sub>8</sub>, Molecular weight 262,2g.mol<sup>-1</sup> | ||
::::: Aliquots of 25 mg | ::::: Aliquots of 25 mg | ||
::::* '''Cell permeable compound [[NV161]]''' | ::::* '''Cell permeable compound [[NV161/MitoKit-CII]]''' | ||
::::: Description: Malonate prodrug: C<sub>9</sub>H<sub>12</sub>O<sub>8</sub>, Molecular weight 248,2g.mol<sup>-1</sup> | ::::: Description: Malonate prodrug: C<sub>9</sub>H<sub>12</sub>O<sub>8</sub>, Molecular weight 248,2g.mol<sup>-1</sup> | ||
| Line 28: | Line 28: | ||
::::* Reference: | ::::* Reference: | ||
:::::: Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial | :::::: Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial Complex I deficiency. Nat Commun 7:12317. [[Ehinger 2016 Nat Commun|»Bioblast link«]] - Original publication introducing Mito-CII. | ||
=== Preparation === | === Preparation === | ||
:::: Both compounds are dissolved in DMSO | :::: Both compounds are dissolved in DMSO and stored in aliquots at -20 °C. | ||
:::: We recommend the following dilutions for reaching the desired concentration in the experiments: | :::: We recommend the following dilutions for reaching the desired concentration in the experiments: | ||
::::* NV118: 100 mM stock solution | ::::* NV118/MitoKit-CII: 100 mM stock solution | ||
::::* NV161: 10 mM stock solution | ::::* NV161/MitoKit-CII: 10 mM stock solution | ||
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Prepared: | Prepared: | ||
::::* In DMSO solution | ::::* In DMSO solution | ||
::::::* at room temperature: 24 hours | ::::::* at room temperature: 24 hours | ||
::::::* at - | ::::::* at -20 °C: for weeks | ||
CAS-Numbers: | CAS-Numbers: | ||
::::* | ::::* NV118/MitoKit-CII: 1592635-33-0 | ||
::::* | ::::* NV161/MitoKit-CII: 1432752-62-9 | ||
Revision as of 17:09, 28 March 2019
MitoKit-CII
| Description | Cell permeable compound - MitoKit-CII, 2 vials; 1 vial of NV118 (25 mg) + 1 vial of NV161 (10 mg) |
|---|---|
| Product ID | 60200-01 |
| Type | O2k |
| Link | MitoKit-CII MitoPedia |
| Image |
Compounds
The MitoKit-CII consists of the first generation of NeuroVive Pharmaceutical AB's cell permeable succinate and malonate prodrugs. It was developed to stimulate (succinate) or inhibit (malonate) the Complex II of the respiratory electron transfer system in intact cells, and will expand the toolbox for evaluating the role of mitochondria in disease.
Compounds of MitoKit-CII
- The MitoKit-CII is composed of the following compounds:
- Cell permeable compound NV118/MitoKit-CII
- Description: Succinate prodrug: C10H14O8, Molecular weight 262,2g.mol-1
- Aliquots of 25 mg
- Cell permeable compound NV161/MitoKit-CII
- Description: Malonate prodrug: C9H12O8, Molecular weight 248,2g.mol-1
- Aliquots of 10 mg
- Reference:
- Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial Complex I deficiency. Nat Commun 7:12317. »Bioblast link« - Original publication introducing Mito-CII.
Preparation
- Both compounds are dissolved in DMSO and stored in aliquots at -20 °C.
- We recommend the following dilutions for reaching the desired concentration in the experiments:
- NV118/MitoKit-CII: 100 mM stock solution
- NV161/MitoKit-CII: 10 mM stock solution
Expiry date of MitoKit-CII compounds (according to NeuroVive)
Before preparation:
- 3 years in -80° C
- 1 year in -20° C
Prepared:
- In DMSO solution
- at room temperature: 24 hours
- at -20 °C: for weeks
CAS-Numbers:
- NV118/MitoKit-CII: 1592635-33-0
- NV161/MitoKit-CII: 1432752-62-9
Labels: MiParea: Instruments;methods
HRR: O2k-Protocol
O2k-chemicals and media
