Difference between revisions of "MitoKit-CII"

Revision as of 09:55, 13 May 2019

MitoKit-CII

Description	Cell permeable compound - MitoKit-CII, 2 vials; 1 vial of plasma membrane-permeable succinate (NV118; 25 mg) and 1 vial of plasma membrane-permeable malonate (NV161; 10 mg).
Product ID	60200-01
Type	O2k
Link	MitoKit-CII MitoPedia
Image

Compounds of MitoKit-CII

The MitoKit-CII consists of the first generation of NeuroVive Pharmaceutical AB's cell permeable succinate and malonate prodrugs. It was developed to stimulate (succinate) or inhibit (malonate) the Complex II of the respiratory electron transfer system in intact cells, and will expand the toolbox for evaluating the role of mitochondria in disease.

The MitoKit-CII is composed of the following compounds:

Cell permeable compound NV118/MitoKit-CII

Description: Succinate prodrug: C₁₀H₁₄O₈, Molecular weight 262.2 g.mol^-1

Aliquots of 25 mg

Cell permeable compound NV161/MitoKit-CII

Description: Malonate prodrug: C₉H₁₂O₈, Molecular weight 248.2 g.mol^-1

Aliquots of 10 mg

Reference

Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial Complex I deficiency. Nat Commun 7:12317. »Bioblast link« - Original publication introducing Mito-CII.

Expiry date of MitoKit-CII compounds (according to NeuroVive)

Before preparation

3 years in -80° C
1 year in -20° C

Prepared

In DMSO solution

at room temperature: 24 h
at -20 °C: for 6 months

CAS-Numbers

NV118/MitoKit-CII: 1592635-33-0
NV161/MitoKit-CII: 1432752-62-9

Labels: MiParea: Instruments;methods

HRR: O2k-Protocol

O2k-chemicals and media

@@ Line 1: / Line 1: @@
 {{Product
-|description=Cell permeable compound - MitoKit-CII, 2 vials; 1 vial of NV118 (25 mg) + 1 vial of NV161 (10 mg)
+|description=Cell permeable compound - MitoKit-CII, 2 vials; 1 vial of plasma membrane-permeable succinate (NV118; 25 mg) and 1 vial of plasma membrane-permeable malonate (NV161; 10 mg).
 |product id=60200-01
 |product type=O2k
@@ Line 17: / Line 17: @@
 ::::* '''Cell permeable compound [[NV118/MitoKit-CII]]'''
-::::: Description: Succinate prodrug: C<sub>10</sub>H<sub>14</sub>O<sub>8</sub>, Molecular weight 262,2g.mol<sup>-1</sup>
+::::: Description: Succinate prodrug: C<sub>10</sub>H<sub>14</sub>O<sub>8</sub>, Molecular weight 262.2 g.mol<sup>-1</sup>
 ::::: Aliquots of 25 mg
@@ Line 23: / Line 23: @@
 ::::* '''Cell permeable compound [[NV161/MitoKit-CII]]'''
-::::: Description: Malonate prodrug: C<sub>9</sub>H<sub>12</sub>O<sub>8</sub>, Molecular weight 248,2g.mol<sup>-1</sup>
+::::: Description: Malonate prodrug: C<sub>9</sub>H<sub>12</sub>O<sub>8</sub>, Molecular weight 248.2 g.mol<sup>-1</sup>
 ::::: Aliquots of 10 mg
-::::* Reference:
+::::* Reference
-:::::: Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial Complex I deficiency. Nat Commun 7:12317. [[Ehinger 2016 Nat Commun|»Bioblast link«]] - Original publication introducing Mito-CII.
+:::::: Ehinger JK, Piel S, Ford R, Karlsson M, Sjövall F, Frostner EÅ, Morota S, Taylor RW, Turnbull DM, Cornell C, Moss SJ, Metzsch C, Hansson MJ, Fliri H, Elmér E (2016) Cell-permeable succinate prodrugs bypass mitochondrial Complex I deficiency. Nat Commun 7:12317. [[Ehinger 2016 Nat Commun|»Bioblast link«]] - ''Original publication introducing Mito-CII''.
@@ Line 34: / Line 34: @@
-Before preparation:
+::: Before preparation
 ::::* 3 years in -80° C
 ::::* 1 year in -20° C
-Prepared:
+::: Prepared
 ::::* In DMSO solution
-::::::* at room temperature: 24 hours
+::::::* at room temperature: 24 h
 ::::::* at -20 °C: for 6 months
-CAS-Numbers:
+::: CAS-Numbers
 ::::* NV118/MitoKit-CII: 1592635-33-0
 ::::* NV161/MitoKit-CII: 1432752-62-9