Difference between revisions of "MitoKit-CII MitoPedia"
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== Application in [[HRR]] == | == Application in [[HRR]] == | ||
=== Preparation === | |||
:::: Both compounds are dissolved in DMSO for 1M concentration stock solution and stored in aliquots at -20. | |||
:::: We recommend the following dilutions for reaching the desired concentration in the experiments: | |||
::::* NV118: 100 mM stock solution | |||
::::* NV161: 10 mM stock solution | |||
== Expiry date of MitoKit-CII compounds (according to NeuroVive) == | |||
Before preparation: | |||
::::* 3 years in -80° C | |||
::::* 1 year in -20° C | |||
Prepared: | |||
::::* In DMSO solution | |||
::::::* at room temperature: 24 hours. | |||
::::::* at -20° C: for weeks | |||
Revision as of 15:51, 25 March 2019
- high-resolution terminology - matching measurements at high-resolution
MitoKit-CII MitoPedia
Description
Cell permeable prodrugs, composed of NV161 and NV118, of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres.
Abbreviation: MitoKit-CII
Reference: MitoKit-CII, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
- For preparation, see MitoKit-CII.
Application in HRR
Preparation
- Both compounds are dissolved in DMSO for 1M concentration stock solution and stored in aliquots at -20.
- We recommend the following dilutions for reaching the desired concentration in the experiments:
- NV118: 100 mM stock solution
- NV161: 10 mM stock solution
Expiry date of MitoKit-CII compounds (according to NeuroVive)
Before preparation:
- 3 years in -80° C
- 1 year in -20° C
Prepared:
- In DMSO solution
- at room temperature: 24 hours.
- at -20° C: for weeks
