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Difference between revisions of "MitoKit-CII MitoPedia"

From Bioblast
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{{MitoPedia
{{MitoPedia
|abbr=MitoKit-CII
|abbr=MitoKit-CII
|description='''Cell permeable prodrugs''', composed of [[NV161]] and [[NV118]], of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres.
|description='''Cell permeable prodrugs''', composed of [[NV161/MitoKit-CII]] and [[NV118/MitoKit-CII]], of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres.
|info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]]
|info=[[MitoKit-CII]], [[Ehinger 2016 Nat Commun]]
}}
}}
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== Application in [[HRR]] ==
== Application in [[HRR]] ==


=== Preparation === Β 
=== Preparation === Β 


:::: Both compounds are dissolved in DMSO for 1M concentration stock solution and stored in aliquots at -20. Β 
:::: Both compounds are dissolved in DMSO and stored in aliquots at -20 Β°C. Β 
:::: We recommend the following dilutions for reaching the desired concentration in the experiments:
:::: We recommend the following dilutions for reaching the desired concentration in the experiments:




::::* NV118: 100 mM stock solution
::::* NV118/MitoKit-CII: 100 mM stock solution
::::* NV161: 10 mM stock solution
::::* NV161/MitoKit-CII: 10 mM stock solution
Β 
Β 
== Expiry date of MitoKit-CII compounds (according to NeuroVive) ==
Β 
Β 
Before preparation:
::::* 3 years in -80Β° C
::::* 1 year in -20Β° C
Β 
Β 
Prepared:
::::* In DMSO solution
::::::* at room temperature: 24 hours.
::::::* at -20Β° C: for weeks

Revision as of 16:12, 28 March 2019


high-resolution terminology - matching measurements at high-resolution


MitoKit-CII MitoPedia

Description

Cell permeable prodrugs, composed of NV161/MitoKit-CII and NV118/MitoKit-CII, of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres.

Abbreviation: MitoKit-CII

Reference: MitoKit-CII, Ehinger 2016 Nat Commun


MitoPedia topics: Media for respirometry 


Application in HRR

Preparation

Both compounds are dissolved in DMSO and stored in aliquots at -20 Β°C.
We recommend the following dilutions for reaching the desired concentration in the experiments:


  • NV118/MitoKit-CII: 100 mM stock solution
  • NV161/MitoKit-CII: 10 mM stock solution