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Difference between revisions of "MitoKit-CII MitoPedia"

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== Specific SUIT protocols ==


*Β  [[SUIT-003_O2_ce_D060]] for living cells using [[MitoKit-CII/Succinate-nv]] and [[MitoKit-CII/Malonate-nv]].


== Application in [[HRR]] ==
== Application in [[HRR]] ==

Revision as of 14:39, 20 June 2019


high-resolution terminology - matching measurements at high-resolution


MitoKit-CII MitoPedia

Description

Cell permeable prodrugs, composed of MitoKit-CII/Succinate-nv and MitoKit-CII/Malonate-nv, stimulates (Snv) or inhibits (Mnanv) mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres, acting on Complex II of the electron transfer system.

Abbreviation: MitoKit-CII

Reference: MitoKit-CII, Ehinger 2016 Nat Commun


MitoPedia topics: Media for respirometry 


Specific SUIT protocols

Application in HRR

Preparation

Both compounds are dissolved in DMSO and stored in aliquots at -20 Β°C.
We recommend the following dilutions for reaching the desired concentration in the experiments:



SUITbrowser question: Permeable succinate/malonate

Work in progress