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Difference between revisions of "MitoKit-CII MitoPedia"

From Bioblast
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== SUITbrowser question: Permeable succinate/malonate ==
== [[SUITbrowser]] question: Permeable succinate/malonate ==
:::: The cell permeable prodrugs Succinate-nv and Malonate-nv can help to answer questions related to the S-pathway in [[living cells]]. Use the [https://suitbrowser.oroboros.at/ SUITbrowser] to find the best protocol to answer this and other research questions.
:::: The cell permeable prodrugs Succinate-nv and Malonate-nv can help to answer questions related to the S-pathway in [[living cells]]. Use the [https://suitbrowser.oroboros.at/ SUITbrowser] to find the best protocol to answer this and other research questions.

Revision as of 09:33, 2 July 2019


high-resolution terminology - matching measurements at high-resolution


MitoKit-CII MitoPedia

Description

Cell permeable prodrugs, composed of MitoKit-CII/Succinate-nv and MitoKit-CII/Malonate-nv, stimulates (Snv) or inhibits (Mnanv) mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres, acting on Complex II of the electron transfer system.

Abbreviation: MitoKit-CII

Reference: MitoKit-CII, Ehinger 2016 Nat Commun


MitoPedia topics: Media for respirometry 


Specific SUIT protocols

Application in HRR

Preparation

Both compounds are dissolved in DMSO and stored in aliquots at -20 Β°C.
We recommend the following dilutions for reaching the desired concentration in the experiments:



SUITbrowser question: Permeable succinate/malonate

The cell permeable prodrugs Succinate-nv and Malonate-nv can help to answer questions related to the S-pathway in living cells. Use the SUITbrowser to find the best protocol to answer this and other research questions.