- high-resolution terminology - matching measurements at high-resolution
MitoKit-CII MitoPedia
Description
Cell permeable prodrugs, composed of NV161 and NV118, of the Complex II substrate succinate increase ATP-linked mitochondrial respiration in CI-deficient human blood cells, fibroblasts and heart fibres.
Abbreviation: MitoKit-CII
Reference: MitoKit-CII, Ehinger 2016 Nat Commun
MitoPedia topics:
Media for respirometry
- For preparation, see MitoKit-CII.
Application in HRR
Preparation
- Both compounds are dissolved in DMSO for 1M concentration stock solution and stored in aliquots at -20.
- We recommend the following dilutions for reaching the desired concentration in the experiments:
- NV118: 100 mM stock solution
- NV161: 10 mM stock solution
Expiry date of MitoKit-CII compounds (according to NeuroVive)
Before preparation:
- 3 years in -80° C
- 1 year in -20° C
Prepared:
- In DMSO solution
- at room temperature: 24 hours.
- at -20° C: for weeks
