MitoPedia alert - 2020
- Currently Answered Questions from the »Oroboros Ecosystem Agenda«
||Celebrating World Oceans Day|
Would you like to measure the O2 flux that occurs during photosynthesis using the O2k and conduct experiments about primary productivity in aquatic ecosystems? Soon you will be able to with the PB-Module that we are developing as part of the H2020 NextGen-O2k project.
||Did you know that dithionite should not be used to control the oxygen concentration in experiments with biological samples? |
- » Dithionite«
||Did you know that if ET capacity is lower than OXPHOS capacity (P>E) in living cells or mitochondrial preparations, then you have encountered an experimental artefact? The possible triggers of this experimental artefact are: |
- loss of oxidative capacity during the time course of the respirometric assay,
- insufficient uncoupler concentrations,
- over titration of an uncoupler, which inhibits ET capacity,
- inhibition of ET capacity due to the use of high oligomycin (inhibitor of the ATP synthase) concentrations employed to measure LEAK respiration prior ET capacity.
Information from: Gnaiger Erich et al ― MitoEAGLE Task Group (2020) Mitochondrial physiology. Bioenerg Commun 2020.1 »Bioblast link«
Previous MitoPedia Alert 2020
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||Does your sample display a preference for glutamate & malate over pyruvate & malate to support NADH-linked respiration? |
DatLab 7.4 provides a step-by-step guide through SUIT-008 and SUIT-014, which can be used in parallel to evaluate the contribution of different combinations of two NADH-linked respiration substrates.
-»SUIT-008« and »SUIT-014«
||Did you know that oxygen concentration in the O2k-Chamber can be decreased by injecting nitrogen gas? |
- »Nitrogen injection«
||As part of the H2020 SME NextGen-O2k project we are developing a PhotoBiology module that will allow to study the effect of different light intensities on O2 consumption and production.|
|| Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases? |
DatLab 7.4 provides a step-by-step guide through the SUIT-024 as a quality control protocol of the mitochondrial preparation
||The optimum effective digitonin concentration for complete plasma membrane permeabilization without mitochondrial outer membrane damage. |
DatLab 7.4 provides a step-by-step guide through the SUIT-010 protocol to test the optimum digitonin concentration.
||Do you want to search the best SUIT protocol for your research questions? |
- Use the SUITbrowser: »SUITbrowser«
||The standardized coupling control protocol to explore the bioenergetics of living cells |
DatLab 7.4 provides a step-by-step guide through the SUIT-003 protocol and its extensions.
||Explore metabolic profiles and specific modulations of mitochondrial pathways in health and disease with SUIT reference protocols. |
- »RP1« and »RP2«
||Spermidine and other calorie restriction mimetics control autophagy and mitochondrial function. |
||What do metformin, melatonin and other bioactive mitObesity compounds have in common? |
- »MitObesity drugs«
||Did you ever think that units for body mass could be an issue? |
- »Body mass«
||Force and Energy: this we all need in the New Year - and MitoPedia may help to clarify the difference.