Difference between revisions of "Mitochondrial respiration media: comparison"
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|description=Mitochondrial respiratory capacity and control are compared in different '''mitochondrial respiration media''', MiRs, to evaluate the quality of MiRs in preserving mitochondrial function and to harmonize results obtained in various studies using different MiRs. In some cases alterations of the formulation are incorporated to optimize conditions for the simultaneous measurement of multiple parameters, e.g. respiration and [[ROS]] production. | |description=Mitochondrial respiratory capacity and control are compared in different '''mitochondrial respiration media''', MiRs, to evaluate the quality of MiRs in preserving mitochondrial function and to harmonize results obtained in various studies using different MiRs. In some cases alterations of the formulation are incorporated to optimize conditions for the simultaneous measurement of multiple parameters, e.g. respiration and [[ROS]] production. | ||
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From the many media used by various groups the composition of 3 examples is shown: | From the many media used by various groups the composition of 3 examples is shown: | ||
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MiR05: [http://bioblast.at/index.php/MiPNet14.13_Medium-MiR06 MiPNet14.03], [http://bioblast.at/index.php/Gnaiger_2000_Life_in_the_Cold Gnaiger 2000 Life in the Cold] | MiR05: [http://bioblast.at/index.php/MiPNet14.13_Medium-MiR06 MiPNet14.03], [http://bioblast.at/index.php/Gnaiger_2000_Life_in_the_Cold Gnaiger 2000 Life in the Cold] | ||
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{{MitoPedia methods}} | |||
{{MitoPedia O2k and high-resolution respirometry}} | |||
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|mitopedia topic=Medium | |||
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Latest revision as of 13:50, 22 August 2022
- high-resolution terminology - matching measurements at high-resolution
Mitochondrial respiration media: comparison
Description
Mitochondrial respiratory capacity and control are compared in different mitochondrial respiration media, MiRs, to evaluate the quality of MiRs in preserving mitochondrial function and to harmonize results obtained in various studies using different MiRs. In some cases alterations of the formulation are incorporated to optimize conditions for the simultaneous measurement of multiple parameters, e.g. respiration and ROS production.
Abbreviation: MiR
From the many media used by various groups the composition of 3 examples is shown:
Note: Media may be stored frozen at -20°C. After thawing MiRK03 a preciptate was detected, but following 20 minutes of shaking at room temperature the precipitate dissapeared again.
References:
Buffer Z: Perry 2011 Biochem J
MiRK03: Krumschnabel 2014 Methods Mol Biol, modified after Komary 2010 Biochim Biophys Acta
MiR05: MiPNet14.03, Gnaiger 2000 Life in the Cold
MitoPedia topics:
Media for respirometry
