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Difference between revisions of "Moreno-Sanchez 2022 Abstract Bioblast"

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Revision as of 11:22, 16 May 2022

Moreno-SĂĄnchez R, Robledo-Cadena DX, Pacheco-VelĂĄzquez SC, RodrĂ­guez-EnrĂ­quez S (2022) Estimation of energy pathway fluxes in cancer cells- beyond the Warburg effect. Bioblast 2022: BEC Inaugural Conference.

Link: Bioblast 2022: BEC Inaugural Conference

Moreno-Sanchez Rafael, Robledo-Cadena Diana Xochiquetzal, Pacheco-Velazquez Silvia Cecilia, Rodriguez-Enriquez Sara (2022)

Event: Bioblast 2022

The experimental determination of the glycolytic and oxidative phosphorylation (OXPHOS) fluxes in cancer and non-cancer cells is analyzed. The steady-state energy metabolism fluxes are in turn used to estimate their respective contributions to the cellular ATP supply. The rate of lactate production, corrected for the fraction generated by glutaminolysis, is proposed as the more appropriate way to estimate glycolysis flux. Indeed, the glycolysis rates estimated for cancer cells are higher than those found in non-cancer cells, as originally observed by Otto Warburg. The rate of total cellular O2 consumption corrected by using oligomycin, a specific, potent and permeable ATP synthase inhibitor, is proposed as the more appropriate way to estimate OXPHOS flux. Detecting non-negligible O2 consumption rates in cancer cells has revealed that the mitochondrial function is not impaired, as claimed by the Warburg effect. Furthermore, when calculating the relative contributions to the cellular ATP supply, under a variety of environmental conditions and for several different types of cancer cells, it is found that OXPHOS is the main ATP provider over glycolysis. Hence, it is shown that to block ATP-dependent processes such as cellular migration in cancer cells, OXPHOS inhibitors can be successfully used. These observations may guide the re-design of novel targeted therapies


‱ O2k-Network Lab: MX Mexiko City Moreno-Sanchez R


Affiliations

Moreno-SĂĄnchez Rafael(1*), Robledo-Cadena Diana Xochiquetzal(2), Pacheco-VelĂĄzquez Silvia Cecilia(2), RodrĂ­guez-EnrĂ­quez Sara(3*)
  1. Laboratorio de Control Metabólico. Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México. Los Reyes Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Árboles/Barrio de los Héroes, Tlalnepantla 54090. Mexico
  2. Departamento de BioquĂ­mica, Instituto Nacional de CardiologĂ­a, Ciudad de MĂ©xico 14080. Mexico
  3. Laboratorio de Control Metabólico. Carrera de Medicina. Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México. Los Reyes Ixtacala, Hab. Los Reyes Ixtacala Barrio de los Árboles/Barrio de los Héroes, Tlalnepantla 54090. Mexico


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