Mu 2023 STAR Protoc

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Mu C, Shearer J (2023) Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats.

Β» STAR Protoc 4:102560. PMID: 37742174 Open Access

Mu Chunlong, Shearer Jane (2023) STAR Protoc

Abstract: Mitochondrial respirometry allows for the comprehensive study of oxygen consumption within the electron transport system in tissues. However, limited techniques exist for analyzing frozen or biobanked intestinal tissues. Here, we present a protocol to evaluate the respiratory function of mitochondria in colonic tissues after cryopreservation at -80Β°C. We describe steps for rat dissection, respirometry calibration, and tissue preparation. We then detail measurement of oxygen respiration and protein concentration. This protocol facilitates the retrospective analysis of mitochondrial respiration in frozen tissue. β€’ Keywords: Health Sciences, Metabolism, Model Organisms, Molecular Biology β€’ Bioblast editor: Plangger M β€’ O2k-Network Lab: CA Calgary Shearer J

Labels: MiParea: Respiration, Instruments;methods 

Stress:Cryopreservation  Organism: Rat  Tissue;cell: Endothelial;epithelial;mesothelial cell  Preparation: Homogenate 

Coupling state: LEAK, ET  Pathway: N, S, NS, ROX  HRR: Oxygraph-2k 


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