Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Myxothiazol

From Bioblast
Revision as of 10:55, 22 June 2021 by Spitzer Gudrun (talk | contribs) (replaced 99.9 % ethanol by ethanol abs.)


high-resolution terminology - matching measurements at high-resolution


Myxothiazol

Description

Myxothiazol, Myx, is an inhibitor of Complex III (CIII). CIII also inhibits CI (G. Lenaz). Myxothiazol binds to the Qo site of CIII (close to cytochrome bL) and inhibits the transfer of electrons from reduced QH2 to the Rieske iron sulfur protein.

Abbreviation: Myx

Reference: MiPNet09.12

Application in HRR

Myx: Myxothiazol (from Myxobacterium myxococcus fulvus; C25H33N3O3S2), Sigma-Aldrich: T5580, store at 4 °C, CAS: 76706-55-3, M = 487.7 g·mol-1
Hazard statements: H300; fatal if swallowed
Available again from Sigma with this new product number. The old product number was M 5779.


Preparation of 1 mM storage and stock solution (dissolved in ethanol abs.) for 2-mL O2k-chamber:
  1. Weigh 1 mg of myxothiazol into a small glass vial.
  2. Add 2.05 mL ethanol.
  3. Divide into 0.2 mL (use glass vials).
  4. Store at -20 °C.
»O2k manual titrations MiPNet09.12
  • Titration volume (2-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
  • Final concentration: 0.5 µM.


Preparation of 0.25mM stock solution (dissolved in ethanol abs.) for 0.5-mL O2k-chamber:
  1. Take 100 µL of the 1 mM stock solution.
  2. Add 300 µL ethanol.
»O2k manual titrations MiPNet09.12
  • Titration volume (0.5-mL O2k-chamber): 1 µL using a 10 µL Hamilton syringe.
  • Final concentration: 0.5 µM.

Compare

  • Antimycin A is less expensive than Myx but induces higher ROS production.







MitoPedia topics: Inhibitor