https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&feed=atom&action=history
Napa 2017 Int J Dent - Revision history
2024-03-29T16:01:27Z
Revision history for this page on the wiki
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https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&diff=224464&oldid=prev
Gnaiger Erich at 09:46, 9 February 2022
2022-02-09T09:46:54Z
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<td colspan="2" style="background-color: #fff; color: #202122; text-align: center;">Revision as of 09:46, 9 February 2022</td>
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from ''P. gingivalis'' negatively alters gingival cell mitochondrial bioenergetics. Int J Dent 2017:2697210.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|title=Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from ''P. gingivalis'' negatively alters gingival cell mitochondrial bioenergetics. Int J Dent 2017:2697210.</div></td></tr>
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&diff=214257&oldid=prev
Cardoso Luiza at 14:44, 25 January 2021
2021-01-25T14:44:40Z
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Cardoso Luiza
https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&diff=213945&oldid=prev
Cardoso Luiza at 16:47, 21 January 2021
2021-01-21T16:47:18Z
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Cardoso Luiza
https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&diff=195442&oldid=prev
Gnaiger Erich at 15:09, 4 March 2020
2020-03-04T15:09:26Z
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Gnaiger Erich
https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&diff=145693&oldid=prev
Kandolf Georg at 13:28, 13 November 2017
2017-11-13T13:28:54Z
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Kandolf Georg
https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&diff=140222&oldid=prev
Krumschnabel Gerhard at 07:57, 1 August 2017
2017-08-01T07:57:48Z
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<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>LPS elicits an increase in gingival cell mitochondria content, with a subsequent increase in reactive oxygen species production (i.e. H<sub>2</sub>O<sub>2</sub>), despite a paradoxical reduction in ATP generation. These findings provide insight into the nature of oxidative stress in oral inflammatory pathologies.</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>LPS elicits an increase in gingival cell mitochondria content, with a subsequent increase in reactive oxygen species production (i.e. H<sub>2</sub>O<sub>2</sub>), despite a paradoxical reduction in ATP generation. These findings provide insight into the nature of oxidative stress in oral inflammatory pathologies.</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|keywords=Mitochondria, Respiration, LPS, Oral gingiva, Oxidative stress</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|keywords=Mitochondria, Respiration, LPS, Oral gingiva, Oxidative stress<ins style="font-weight: bold; text-decoration: none;">; HGF-1 human gingival fibroblast</ins></div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]],</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|editor=[[Kandolf G]],</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=US UT Provo Bikman BT</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|mipnetlab=US UT Provo Bikman BT</div></td></tr>
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<td colspan="2" class="diff-lineno">Line 24:</td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|couplingstates=LEAK, OXPHOS, ETS</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|couplingstates=LEAK, OXPHOS, ETS</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|pathways=N, NS, ROX</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>|pathways=N, NS, ROX</div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|instruments=Oxygraph-2k<ins style="font-weight: bold; text-decoration: none;">, O2k-Fluorometer</ins></div></td></tr>
<tr><td class="diff-marker" data-marker="−"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #ffe49c; vertical-align: top; white-space: pre-wrap;"><div>|additional=<del style="font-weight: bold; text-decoration: none;">Labels, </del>2017-06, Magnesium Green, Amplex UltraRed,</div></td><td class="diff-marker" data-marker="+"></td><td style="color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #a3d3ff; vertical-align: top; white-space: pre-wrap;"><div>|additional=2017-06, Magnesium Green, Amplex UltraRed,</div></td></tr>
<tr><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td><td class="diff-marker"></td><td style="background-color: #f8f9fa; color: #202122; font-size: 88%; border-style: solid; border-width: 1px 1px 1px 4px; border-radius: 0.33em; border-color: #eaecf0; vertical-align: top; white-space: pre-wrap;"><div>}}</div></td></tr>
</table>
Krumschnabel Gerhard
https://wiki.oroboros.at/index.php?title=Napa_2017_Int_J_Dent&diff=137315&oldid=prev
Kandolf Georg: Created page with "{{Publication |title=Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from ''P. gingiv..."
2017-06-14T13:34:36Z
<p>Created page with "{{Publication |title=Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from ''P. gingiv..."</p>
<p><b>New page</b></p><div>{{Publication<br />
|title=Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT (2017) LPS from ''P. gingivalis'' negatively alters gingival cell mitochondrial bioenergetics. Int J Dent 2017:2697210.<br />
|info=[https://www.ncbi.nlm.nih.gov/pubmed/28592970 Open Access]<br />
|authors=Napa K, Baeder AC, Witt JE, Rayburn ST, Miller MG, Dallon BW, Gibbs JL, Wilcox SH, Winden DR, Smith JH, Reynolds PR, Bikman BT<br />
|year=2017<br />
|journal=Int J Dent<br />
|abstract=Oral inflammatory pathologies are linked to increased oxidative stress, thereby partly explaining their relevance in the etiology of systemic disorders. The purpose of this work was to determine the degree to which LPS from ''Porphyromonas gingivalis'', the primary pathogen related to oral inflammation, altered gingival mitochondrial function and reactive oxygen species generation. <br />
<br />
Human gingival fibroblast (HGF-1) cells were treated with lipopolysaccharide of ''P. gingivalis''. Mitochondrial function was determined via high-resolution respirometry.<br />
<br />
LPS-treated HGF-1 cells had significantly more mitochondrial complex IV and higher rates of mitochondrial respiration. However, this failed to translate into greater ATP production, as ATP production was paradoxically diminished with LPS treatment. Nevertheless, production of the reactive H<sub>2</sub>O<sub>2</sub> was elevated with LPS treatment.<br />
<br />
LPS elicits an increase in gingival cell mitochondria content, with a subsequent increase in reactive oxygen species production (i.e. H<sub>2</sub>O<sub>2</sub>), despite a paradoxical reduction in ATP generation. These findings provide insight into the nature of oxidative stress in oral inflammatory pathologies.<br />
|keywords=Mitochondria, Respiration, LPS, Oral gingiva, Oxidative stress<br />
|editor=[[Kandolf G]],<br />
|mipnetlab=US UT Provo Bikman BT<br />
}}<br />
{{Labeling<br />
|area=Respiration, Pharmacology;toxicology<br />
|organism=Human<br />
|tissues=Endothelial;epithelial;mesothelial cell<br />
|preparations=Permeabilized cells<br />
|topics=ATP production<br />
|couplingstates=LEAK, OXPHOS, ETS<br />
|pathways=N, NS, ROX<br />
|instruments=Oxygraph-2k<br />
|additional=Labels, 2017-06, Magnesium Green, Amplex UltraRed,<br />
}}</div>
Kandolf Georg