Cookies help us deliver our services. By using our services, you agree to our use of cookies. More information

Difference between revisions of "NextGen-O2k Documentation"

From Bioblast
(12 intermediate revisions by 5 users not shown)
Line 14: Line 14:
:::: In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.
:::: In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.


== Achievements ==


::::'''Project Year 1:''' In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.
::::'''Project Year 2:''' Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module.


== Progress and next steps ==
== Progress and next steps ==


:::: '''2019-12-06:''' First version of the CV-Manual released [[MiPNet24.16 DatLab8.0: CV-Module]]
:::: See » [[Talk:NextGen-O2k Documentation|Track record]]
:::: '''2019-11-22:''' First draft of NextGen-O2k Manual/SOP finalized
=== [[Image:PlayVideo.jpg|50px|link=https://wiki.oroboros.at/index.php/O2k-Videosupport]] NextGen-O2k Videosupport clips  ===
:::: '''2019-10-21:''' Videos for our software DatLab 7.4 (how to find a DL-Protocol: MitoPedia and SUITbrowser, how to perform an experiment, O2 flux analysis with DatLab 7.4 and export DL-Protocol User)
:::: '''2019-09-24:''' Videos for mitochondrial preparations are released – check them out: [https://wiki.oroboros.at/index.php/O2k-Videosupport#O2k-Mitochondrial_preparations O2k-Videosupport Mitochondrial preparations]
:::: '''2019-09-20:''' First videos for the Q-Module are released – check them out: [https://wiki.oroboros.at/index.php/O2k-Videosupport#Q-Module_.28NextGen-O2k_-_DatLab_7.29 O2k-Videosupport Q-Module]
:::: '''2019-09-02:''' Recording of videos for our NextGen-O2k  
:::: '''2019-06-17:''' Our Marketing Thaumaturge [[Cocco Paolo|Paolo Cocco]] participates at IOC139 Schroecken AT - to start with the recording of the first videos


[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible mw-collapsed">
'''Q-Module (NextGen-O2k - DatLab 7)'''


== Links and references ==
<div class="mw-collapsible-content">
 
* '''Q-service'''
 
[https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be  Assembly of the reference electrode]
[https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]
[https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]
 
---------
 
* ''' Q-experiments'''
[https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments]
 
---------
 
* ''' Cleaning Q-Sensors'''
[https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]
 
[https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments]
 
</div>
</div>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible mw-collapsed">
'''FluoRespirometry'''
<div class="mw-collapsible-content">
 
 
[https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]
 
[https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the smart-fluo sensor]
 
[https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4]
 
</div>
</div>
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible mw-collapsed">
'''O2k-Mitochondrial preparations'''
<div class="mw-collapsible-content">
 
[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]
 
[https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be  Fiber permeabilization]
[https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber]
 
</div>
</div>
 
[[File:Expand.png|right|45px |Click to expand or collaps]]
<div class="toccolours mw-collapsible mw-collapsed">
'''Quality Control'''
<div class="mw-collapsible-content">
 
[https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration]
</div>
</div>
 
=== [[Image:O2k-Manual.jpg|50px|link=https://wiki.oroboros.at/index.php/O2k-Manual]] NextGen-O2k Manuals ===
 
::: » [[MiPNet24.16 DatLab8.0: CV-Module|DatLab8.0: CV-Module]]
 
::: » [[MiPNet24.12 NextGen-O2k: Q-Module |NextGen-O2k: Q-Module]]
 
::: » [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4| Oxygen flux_analysis - DatLab 7.4]]
 
::: » [[MiPNet24.08 Safranin Analysis Template| Safranin Analysis Template]]
 
::: » [[MiPNet24.09 General Template for Mt-membrane Potential Analysis| General template for mt-membrane potential analysis]]
 
::: » [[MiPNet24.10 H2O2 flux analysis| H<sub>2</sub>O<sub>2</sub> flux analysis]]

Revision as of 11:20, 6 May 2021

Template:MitoFit highlights page name

NextGen-O2k

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP3: Documentation



Abstract

In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.

Achievements

Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.
Project Year 2: Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module.

Progress and next steps

See » Track record

PlayVideo.jpg NextGen-O2k Videosupport clips

Click to expand or collaps
Click to expand or collaps
Click to expand or collaps
Click to expand or collaps


O2k-Manual.jpg NextGen-O2k Manuals

» DatLab8.0: CV-Module
» NextGen-O2k: Q-Module
» Oxygen flux_analysis - DatLab 7.4
» Safranin Analysis Template
» General template for mt-membrane potential analysis
» H2O2 flux analysis