Difference between revisions of "NextGen-O2k Documentation"
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::::'''Project Year 1:''' In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes. | ::::'''Project Year 1:''' In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes. | ||
::::'''Project Year 2:''' Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module. These materials provide step-by-step instructions, support and information for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published. | |||
== Progress and next steps == | == Progress and next steps == | ||
:::: | :::: See » [[Talk:NextGen-O2k Documentation|Track record]] | ||
=== [[Image:PlayVideo.jpg|50px|link=https://wiki.oroboros.at/index.php/O2k-Videosupport]] NextGen-O2k Videosupport clips === | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible mw-collapsed"> | |||
'''Q-Module (NextGen-O2k - DatLab 7)''' | |||
== | <div class="mw-collapsible-content"> | ||
* '''Q-service''' | |||
[https://www.youtube.com/watch?v=LlIKDFDDeyQ&feature=youtu.be Assembly of the reference electrode]<br> | |||
[https://www.youtube.com/watch?v=EOra9oYgbmU&feature=youtu.be NG Polishing glassy carbon and platinum electrodes]<br> | |||
[https://www.youtube.com/watch?v=vFmHBevN8f4&feature=youtu.be NG O2k-Chamber volume calibration with Q-Sensor]<br> | |||
--------- | |||
* ''' Q-experiments''' | |||
[https://www.youtube.com/watch?v=K10BJZHMDpE&feature=youtu.be NG Q-experiments] | |||
--------- | |||
* ''' Cleaning Q-Sensors''' | |||
[https://www.youtube.com/watch?v=D9PaFcB_yKk&feature=youtu.be NG Cleaning after cyclic voltammetry]<br> | |||
[https://www.youtube.com/watch?v=-VXGTVc3ywA&feature=youtu.be NG Cleaning after Q-experiments] | |||
</div> | |||
</div> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible mw-collapsed"> | |||
'''FluoRespirometry''' | |||
<div class="mw-collapsible-content"> | |||
[https://www.youtube.com/watch?v=nlVLXcbBRqs Smart Fluo-Sensor assembly]<br> | |||
[https://www.youtube.com/watch?v=eUukvgAi1yY Exchanging a filter set of the smart-fluo sensor]<br | |||
>[https://www.youtube.com/watch?v=_Fv0VSA_c14 H<sub>2</sub>O<sub>2</sub> flux analysis with DatLab 7.4] | |||
</div> | |||
</div> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible mw-collapsed"> | |||
'''O2k-Mitochondrial preparations''' | |||
<div class="mw-collapsible-content"> | |||
[https://www.youtube.com/watch?v=EjQGUr5M8v0&feature=youtu.be Shredder tissue homogenate]<br> | |||
[https://www.youtube.com/watch?v=iN38rOkiBUQ&feature=youtu.be Fiber permeabilization]<br> | |||
[https://www.youtube.com/watch?v=GX_SvnVGXgg Addition of biological sample to the respirometer chamber] | |||
</div> | |||
</div> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible mw-collapsed"> | |||
'''Quality Control''' | |||
<div class="mw-collapsible-content"> | |||
[https://www.youtube.com/watch?v=onOIVpmOEoE O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration] | |||
</div> | |||
</div> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible mw-collapsed"> | |||
'''NextGen-O2k trailers''' | |||
<div class="mw-collapsible-content"> | |||
[https://www.youtube.com/watch?v=MQGa_xMkyA4 NextGen-O2k: the Q-Module]<br> | |||
[https://www.youtube.com/watch?v=2UQ4ffzUz_o NextGen-O2k: the NADH-Module]<br> | |||
[https://www.youtube.com/watch?v=UEpuz7mbbBQ NextGen-O2k: the PQ-Module]<br> | |||
</div> | |||
</div> | |||
[[File:Expand.png|right|45px |Click to expand or collaps]] | |||
<div class="toccolours mw-collapsible mw-collapsed"> | |||
'''NextGen-O2k demo''' | |||
<div class="mw-collapsible-content"> | |||
* NextGen-O2k demo: Coenzyme Q redox state and mt-respiration<br> | |||
* NextGen-O2k demo: mt-respiration and NADH<br> | |||
* NextGen-O2k demo: mt-respiration and H<sub>2</sub>O<sub>2</sub><br> | |||
* NextGen-O2k demo: H<sub>2</sub>O<sub>2</sub> calibration<br> | |||
</div> | |||
</div> | |||
=== [[Image:O2k-Manual.jpg|50px|link=https://wiki.oroboros.at/index.php/O2k-Manual]] NextGen-O2k Manuals === | |||
::: » [[MiPNet26.13_NextGen-O2k_manual|NextGen-O2k manual]] | |||
::: » [[MiPNet24.16 DatLab8.0: CV-Module|DatLab8.0: CV-Module]] | |||
::: » [[MiPNet24.12 NextGen-O2k: Q-Module |NextGen-O2k: Q-Module]] | |||
::: » [[MiPNet26.12 NextGen-O2k: NADH-Module|NextGen-O2k: NADH-Module]] | |||
::: » [[MiPNet26.11 NextGen-O2k: PB-Module|NextGen-O2k: PB-Module]] | |||
::: » [[MiPNet06.03 POS-calibration-SOP|O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration - DatLab 8]] | |||
::: » [[MiPNet24.06 Oxygen flux analysis - DatLab 7.4| Oxygen flux_analysis - DatLab 7.4]] | |||
::: » [[MiPNet24.08 Safranin Analysis Template| Safranin Analysis Template]] | |||
::: » [[MiPNet24.09 General Template for Mt-membrane Potential Analysis| General template for mt-membrane potential analysis]] | |||
::: » [[MiPNet24.10 H2O2 flux analysis| H<sub>2</sub>O<sub>2</sub> flux analysis]] | |||
Latest revision as of 13:23, 3 April 2022
NextGen-O2k Documentation
The revolutionary all-in-one instrument to conquer mitochondrial disease.
WP3: Documentation
Abstract
- In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.
Achievements
- Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.
- Project Year 2: Year 2 proved to be a continuation of the development of SOPs and video clips. A PB-Module manual was released for the algae KOLs and new video clips ranged from basic set-up and quality control to more advanced techniques like the DIC saturation curve using the PB Module. These materials provide step-by-step instructions, support and information for using the NextGen-O2k. Furthermore, trailers for the new modules (Q, NADH and PB) were recorded and published.
Progress and next steps
- See » Track record
NextGen-O2k Videosupport clips
Q-Module (NextGen-O2k - DatLab 7)
FluoRespirometry
O2k-Mitochondrial preparations
NextGen-O2k trailers
NextGen-O2k demo
- NextGen-O2k demo: Coenzyme Q redox state and mt-respiration
- NextGen-O2k demo: mt-respiration and NADH
- NextGen-O2k demo: mt-respiration and H2O2
- NextGen-O2k demo: H2O2 calibration
