NextGen-O2k Documentation

The revolutionary all-in-one instrument to conquer mitochondrial disease.


WP3: Documentation

Abstract

In Workpackage 3 a complete set of SOPs, user manuals, tutorial videos and webinars for the NextGen-O2k will be generated. Training courses for customers as well as for staff will be developed and offered.

Achievements

Project Year 1: In year one of the project, we aimed to improve the reproducibility of experimental results and support customers with new developments. Therefore, we created an SOP for the new Q-Module, 6 new manuals and O2k-Videosupport clips for the Q-Module, DatLab7.4, mitochondrial preparations, and TPP+ and ISE electrodes.

Progress and next steps

2021-03-18: Video clip for sample types and mitochondrial preparations is finished.

2021-03-18: Video clip for mitochondrial respiratory control - pathway control is finished.

2021-03-18: Video clip for mitochondrial respiratory control - coupling control is finished.

2021-02-05: Video clip for DIC saturation curve using the PB Module was provided to the KOLs.

2020-11-30: PB-Module manual available for the algae KOLs.

2020-10-27: Video clip for H₂O₂ flux analysis with DatLab 7.4 is finished.

2020-10-27: Video clip for Exchanging a filter set of the Smart-Fluo Sensor is finished.

2020-10-27: Video clip for Smart Fluo-Sensor assembly is finished.

2020-10-27: Video clip for addition of biological sample to the respirometer chamber is finished.

2020-10-27: Video clip for O2k Quality Control 1 is finished.

2020-05-28: Recorded videos for H₂O₂ and mitochondrial membrane potential analysis with DatLab 7.4

2020-02-07: Recorded and prepared videos for quality control steps 1 and 2 (QC1, QC2)

2019-12-06: First version of the CV-Manual released MiPNet24.16 DatLab8.0: CV-Module

2019-11-22: First version of NextGen-O2k Manual/SOP finalized: NextGen-O2k: Q-Module

2019-11-07: Video clips editions for mitochondrial membrane potential with TPP+ and ISE electrodes

2019-10-21: Videos for our software DatLab 7.4 are released - see: O2k-Videosupport DatLab 7.4

2019-09-24: Videos for mitochondrial preparations are released – see: O2k-Videosupport Mitochondrial preparations

2019-09-20: Manual for the oxygen flux analysis with DatLab 7.4: Oxygen flux_analysis - DatLab 7.4

2019-09-20: Manual for data analysis of mitochondrial membrane potential using safranin: Safranin Analysis Template

2019-09-20: Manual for data analysis of mitochondrial membrane potential using various fluorescence dyes: General template for mt-membrane potential analysis

2019-09-20: Manual for hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4: H₂O₂ flux analysis

2019-09-20: First videos for the Q-Module are released – see: O2k-Videosupport Q-Module

2019-09-02: Recording of videos for our NextGen-O2k

2019-06-17: Our Marketing Thaumaturge Paolo Cocco participates at IOC139 Schroecken AT - to start with the recording of the first videos