Difference between revisions of "Nicotinamide adenine dinucleotide"
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Communicated by [[Gnaiger E]] 2016-02-12, edited 2016-11-29. | Communicated by [[Gnaiger E]] 2016-02-12, edited 2016-11-29. | ||
== Application in [[ | == Application in [[HRR]] == | ||
:::: '''Nicotinadeninedinucleotide, NADH''' (β-Nicotinamide adenine dinucleotide, reduced potassium salt, C<sub>21</sub>H<sub>27</sub>N<sub>7</sub>O<sub>14</sub>P<sub>2</sub>K<sub>2</sub>); Sigma A 4505, 100 mg, store at -20 °C (old Sigma recommendation: 4-8 °C); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/n4505pis.pdf]. The same precautions are recommended by Sigma for the sodium salt. | :::: '''Nicotinadeninedinucleotide, NADH''' (β-Nicotinamide adenine dinucleotide, reduced potassium salt, C<sub>21</sub>H<sub>27</sub>N<sub>7</sub>O<sub>14</sub>P<sub>2</sub>K<sub>2</sub>); Sigma A 4505, 100 mg, store at -20 °C (old Sigma recommendation: 4-8 °C); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Product_Information_Sheet/2/n4505pis.pdf]. The same precautions are recommended by Sigma for the sodium salt. | ||
Revision as of 13:45, 17 March 2019
- high-resolution terminology - matching measurements at high-resolution
Nicotinamide adenine dinucleotide
Description
Nicotinamide adenine dinucleotide, NAD+ and NADH (pyridine nucleotide coenzymes, NAD and NADP), is an oxidation-reduction coenzyme (redox cofactor; compare FADH2). In the NADH Electron transfer-pathway state fuelled by type N substrates, mt-matrix dehydrogenases generate NADH, the substrate of Complex I (CI). The mt-NADH pool integrates the activity of the TCA cycle and various matrix dehydrogenases upstream of CI, and thus forms a junction or funnel of electron transfer to CI, the N-junction (compare F-junction, Q-junction). NAD+ and NADH are not permeable through the inner mt-membrane. Cytosolic NADH is effectively made available for mitochondrial respiration through the malate-aspartate shuttle or glycerophosphate dehydrogenase Complex.
Abbreviation: NADH
MitoPedia topics:
Substrate and metabolite
Communicated by Gnaiger E 2016-02-12, edited 2016-11-29.
Application in HRR
- Nicotinadeninedinucleotide, NADH (β-Nicotinamide adenine dinucleotide, reduced potassium salt, C21H27N7O14P2K2); Sigma A 4505, 100 mg, store at -20 °C (old Sigma recommendation: 4-8 °C); FW = 742.61, for important considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [1]. The same precautions are recommended by Sigma for the sodium salt.
Historical terminology
- DPN+ = NAD+
- DPNH, reduced diphosphopyridine nucleotide = NADH
- TPN+ = NADP+
- TPNH, reduced triphosphopyridine nucleotide = NADPH
