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Difference between revisions of "O2k-Catalogue: O2k-Modules"

From Bioblast
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* [[Hydrogen peroxide]]
* [[Hydrogen peroxide]]
* [[Cytochrome Spectra and Oxygen]]
* [[Cytochrome Spectra and Oxygen]]
[[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]].


Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
# Quality control of preparations by respirometric performance.
# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
# Additional information is acquired for a limited amount of sample.
# Oxygen dependence of parameters can be studied.
In the publication by [[Krumschnabel_2015_Methods_Mol_Biol|Krumschnabel et al. 2015]] we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H<sub>2</sub>O<sub>2</sub> production of mouse brain mitochondria. The paper by [[Makrecka-Kuka_2015_Biomolecules|Makrecka-Kuka et al. 2015]] focuses on the application of the Amplex red assay for determining H<sub>2</sub>O<sub>2</sub> production in three different models of mitochondrial preparations.


== '''O2k-Catalogue: O2k-Modules''' ==
== '''O2k-Catalogue: O2k-Modules''' ==

Revision as of 16:06, 15 November 2019

                



O2k-Catalogue: O2k-Modules

O2k-Catalogue

Description 4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.
Product ID 4####
Type O2k, MultiSensor
Link
Image 4####


O2k-Core to FluoRespirometer

O2k-Modules

O2k-Modules are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an O2k-MultiSensor application. The O2k fully supports the O2k-Modules.

High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels.

Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:

  1. Quality control of preparations by respirometric performance.
  2. Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
  3. Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
  4. Additional information is acquired for a limited amount of sample.
  5. Oxygen dependence of parameters can be studied.

In the publication by Krumschnabel et al. 2015 we introduce the O2k-Fluo LED2-Module, describe one selected stage in its development, and show its application for the simultaneous measurement of respiration and H2O2 production of mouse brain mitochondria. The paper by Makrecka-Kuka et al. 2015 focuses on the application of the Amplex red assay for determining H2O2 production in three different models of mitochondrial preparations.

O2k-Catalogue: O2k-Modules

O2k-MultiSensor Manual

ChapterSectionLast update
MiPNet15.03 O2k-MultiSensor-ISE
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2021-08-02
MiPNet15.05 NO-manual
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-Module
O2k-Manual
O2k-Fluo LED2-Module.
2021-06-22
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08
MiPNet24.12 NextGen-O2k: Q-Module
O2k-Manual
NextGen-O2k: Q-Module manual
2021-10-29
MiPNet26.11 NextGen-O2k: PB-Module
O2k-Manual
NextGen-O2k: PB-Module manual
2021-10-29
MiPNet26.12 NextGen-O2k: NADH-Module
O2k-Manual
NextGen-O2k: NADH-Module manual
2022-05-17
MiPNet28.09 O2k-Fluo Smart-Module manual
O2k-Manual
O2k-Fluo Smart-Module manual
2023-09-25
Β»O2k-Publications: O2k-Fluorometry