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Difference between revisions of "O2k-Catalogue: O2k-Modules"

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{{Product
{{Product
|description='''''Catalogue O2k-MultiSensor components'''''
|description='''4'''#### '''''O2k-Catalogue: O2k-Modules'''''. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.
'''O2k-MultiSensor Modules''' are add-on modules supported by the [[O2k-Core]].
|product id=4'''####'''
|product id=4'''####'''
|product type=O2k, MultiSensor, Catalogue
|product type=O2k, MultiSensor,  
|info=Order information: [http://www.oroboros.at/?Purchase @OROBOROS]  /  See also: [http://www.oroboros.at/?O2k-MultiSensor O2k-MultiSensor @OROBOROS]
|product image='''4'''####
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{{OroboroPedia}}
 
== '''Catalogue O2k-MultiSensor components''' ==
[[File:O2k-Core to FluoRespirometer.jpg|right|400px |O2k-Core to FluoRespirometer]]
{{#ask: mainlabel=Title|[[Category:Products]] | [[Product type::MultiSensor]]  
== O2k-Modules ==
 
'''O2k-Modules''' are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an '''O2k-MultiSensor''' application.  The O2k fully supports the O2k-Modules.
* [[MiPNet17.05 O2k-Fluo LED2-Module]]
* [[O2k-TPP%2B_ISE-Module]]
* [[O2k-pH ISE-Module]]
* [[O2k-NO Amp-Module]]
* [[Hydrogen peroxide]]
* [[Cytochrome Spectra and Oxygen]]
[[High-resolution respirometry]] is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: [[O2k-Publications:_O2k-Fluorometry_-_Fluorophores|H<sub>2</sub>O<sub>2</sub> production, mitochondrial membrane potential, intracellular pH, Ca<sup>2+</sup>, Mg<sup>2+</sup>, and NADH levels]].
 
Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:
# Quality control of preparations by respirometric performance.
# Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP<sup>+</sup> or fluorophores.
# Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
# Additional information is acquired for a limited amount of sample.
# Oxygen dependence of parameters can be studied.
 
 
== '''O2k-Catalogue: O2k-Modules''' ==
{{#ask:[[Category:Products]][[Product type::O2k-Module]]  
| ?description
| ?description
| ?product id
| ?product id
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== O2k-MultiSensor ==


When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the [[Oxygraph-2k]], this is an '''O2k-MultiSensor''' application.  The [[O2k-Core]] fully supports the [[O2k-MultiSensor Module]]s.
== O2k-MultiSensor Manual ==


The Oxygraph-2k 20000-44 (Series E), or O2k (Series A-D) requires the [[O2k-MultiSensor electronic upgrade]]. An exception is the application of an NO sensor in a single chamber of the Series A to C Oxygraph-2k.  
{{#ask:[[Category:Publications]] [[Instrument and method::O2k-Manual]] [[Additional label::O2k-MultiSensor]]
| mainlabel=Chapter
|?Has title=Section
|?Was published in year=Last update
|format=broadtable
|limit=500
|sort=
|order=ascending
|offset=0
}}
: »[[O2k-Publications: O2k-Fluorometry]]
== References ==
:::* Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - [[Makrecka-Kuka 2015 Biomolecules |»Bioblast link«]]


See also:
:::* Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - [[Krumschnabel 2015 Methods Mol Biol |»Bioblast link«]]
*[[List of O2k-MultiSensor products]]
*[[O2k-Fluorescence Module]]
O2k-MultiSensor Modules: [[O2k-TPP+ and Ca2+ Electrode Module (ISE)]], [[O2k-pH Electrode Module]], [[O2k-NO MultiSensor Stoppers]]
*[[Mitochondrial membrane potential]]
*[[Calcium electrode]]
*[[Measuring hydrogen peroxide]]
*[[pH electrode]]
*[[NO electrode]]

Latest revision as of 16:10, 15 November 2019

                



O2k-Catalogue: O2k-Modules

O2k-Catalogue

Description 4#### O2k-Catalogue: O2k-Modules. O2k-Modules can be obtained with the O2k or added later, and can be simply installed by the user.
Product ID 4####
Type O2k, MultiSensor
Link
Image 4####


O2k-Core to FluoRespirometer

O2k-Modules

O2k-Modules are add-on modules supported by the O2k. When one (or more) analytical parameters are monitored simultaneously with oxygen concentration and oxygen flux in the O2k, this is an O2k-MultiSensor application. The O2k fully supports the O2k-Modules.

High-resolution respirometry is extended by O2k-MultiSensor modules allowing the simultaneous measurement of oxygen consumption and one additional parameter. Fluorometric methods are available for a wide range of analytical parameters of major interest in mitochochondrial physiology: H2O2 production, mitochondrial membrane potential, intracellular pH, Ca2+, Mg2+, and NADH levels.

Advantages of the simultaneous measurement of additional parameters in a single respirometric chamber:

  1. Quality control of preparations by respirometric performance.
  2. Objective exclusion criteria for protocol related problems, e.g. overtitration of uncouplers, side effects of TPP+ or fluorophores.
  3. Elimination of artifacts of normalization for a mitochondrial marker when combining two methods in a single chamber.
  4. Additional information is acquired for a limited amount of sample.
  5. Oxygen dependence of parameters can be studied.


O2k-Catalogue: O2k-Modules

O2k-MultiSensor Manual

ChapterSectionLast update
MiPNet15.03 O2k-MultiSensor-ISE
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
2021-08-02
MiPNet15.05 NO-manual
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
2016-04-21
MiPNet17.05 O2k-Fluo LED2-Module
O2k-Manual
O2k-Fluo LED2-Module.
2021-06-22
MiPNet23.15 O2k-pH ISE-Module
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
2018-09-08
MiPNet24.12 NextGen-O2k: Q-Module
O2k-Manual
NextGen-O2k: Q-Module manual
2021-10-29
MiPNet26.11 NextGen-O2k: PB-Module
O2k-Manual
NextGen-O2k: PB-Module manual
2021-10-29
MiPNet26.12 NextGen-O2k: NADH-Module
O2k-Manual
NextGen-O2k: NADH-Module manual
2022-05-17
MiPNet28.09 O2k-Fluo Smart-Module manual
O2k-Manual
O2k-Fluo Smart-Module manual
2023-09-25
»O2k-Publications: O2k-Fluorometry

References

  • Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. Biomolecules 5:1319-38. - »Bioblast link«
  • Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61. - »Bioblast link«