Edit MitoPedia: O2k-Fluo LED2-Module

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{{Technical support}} __TOC__ {{MitoPedia without banner |abbr= |description=[[Image:Fluorescence-Control Unit lettered.jpg|180px|right]] The '''O2k-Fluo LED2-Module''' is a component of the O2k-Fluorometer (O2k-Series D to G). It is an amperometric add-on module to the [[O2k-Core]] (O2k-Series D to G), adding a new dimension to high-resolution respirometry. Optical sensors are inserted through the front window of the O2k-glass chambers, for measurement of hydrogen peroxide production (Amplex® UltraRed), ATP production (Magnesium Green™), mt-membrane potential (Safranin, TMRM, Rhodamine 123), Ca²⁺ (Calcium Green™), and numerous other applications open for O2k-user innovation. |info=[https://www.oroboros.at/index.php/product/o2k-fluo-led2-module/ '''Product details and purchase information'''] }} == Scope of application == {{Template:Scope of fluorescence application}} == Select LED Fluo-Sensors == {{Template:Select fluorescence sensor}} == Fluorescence dyes and settings == {{Template:Fluorescence dyes and settings}} == Mounting a filter cap == :::* ''See'': [[Filter-Cap#Mounting_a_Filter-Cap|Mounting a filter cap]] == Stoppers == {{Template:Stoppers for fluorometry}} == Connect LED Fluo-Sensor to O2k == :::# [[Fluorescence-Control_Unit#Setup_of_the_O2k-Fluorescence_LED2-Module|Setup of the O2k-Fluo LED2-Module]] :::# [[Fluorescence-Control_Unit#Power_on|Power on]] == Electronic settings and LED intensity == :::* ''See'': [[Fluorescence-Control_Unit#O2k-Guide|Electronic settings and LED intensity]] == O2k signal and output == ::::# [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]]: The O2k-Fluo LED2-Module is operated through the amperometric (Amp)-Channel of the O2k. ::::# [[O2k signals and output#O2k output |O2k output]]: type I, II, or III, or combinations. == DatLab == [[Image:DL7graph_select_plots_amp.jpg|thumb|250px|alt=Check Amp raw signal form Graph/Select Plots to display the fluorometric signal| Graph / Select plots‎ ]]'''Graph layout''': Three plots are available in DatLab based on the recorded signal: '''Amp Raw Signal''', '''Amp Calibrated''', and '''Amp Slope'''<nowiki>. These plots can be selected from the drop-down lines and displayed with their check boxes either on the Y1 or Y2 [Graph layout / Select Plots].</nowiki> :::: '''Amp Raw Signal''' displays the raw voltage (including amplification) as recorded by the O2k at a given gain setting. :::: '''Amp Calibrated''' is the signal after calibration with the parameters set in the O2k-MultiSensor Calibration window. :::: '''Amp slope''' is the time derivative of the '''calibrated''' signal, multiplied by '''1000''', in units [mV(conc. Unit during calibration)/s], so if the signal was calibrated in µM [nmol/ml] the unit of the slope is pmol/(s ml). To obtain the slope of the raw signal check the appropriate box in the calibration window ([[DatLab]] 5.1.0.130 and above). :::: '''Graphs''' can be generated to display oxygen and fluorescence data, or several graphs can be added to display oxygen and fluorescence data separately. Layout templates are provided, which can be modified and saved as appropriate. All graph settings can be saved as user-defined layouts, see [[MiPNet19.01C DatLab Guide]]. == O2k-Fluorometry and the TIP2k == ::::* [[Titration-Injection microPump |TIP2k]]: Our tests indicated that the fluorometric signal is not affected by the TIP2k needle inserted into the 2-mL O2k-chamber. == Troubleshooting == :::* ''-See'': [[Defective fluorescence module]] == References == [[Image:O2k-Publications.jpg|left|116px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] <br /> <div class="toccolours mw-collapsible mw-collapsed"> ::: <span style="font-size:105%; color:##424242">'''» O2k-Publications'''</span> <div class="mw-collapsible-content"> '''»[[O2k-Publications: Topics]]''' '''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''. Default sorting: chronological. Empty fields appear first in ascending order. {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]] |?Was published in year |?Has title |?Mammal and model=Organism |?Tissue and cell=Tissue;cell |format=broadtable |limit=5000 |offset=0 |sort=Was published in year |order=descending }} </div> </div> <br /> <br /> <div class="toccolours mw-collapsible mw-collapsed"> ::: <span style="font-size:105%; color:##424242">'''» O2k-Publications: O2k-Fluorometry - Abstracts'''</span> <div class="mw-collapsible-content"> '''''Sort in ascending/descending order by a click on one of the small symbols in squares below'''''. {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Instrument and method::O2k-Fluorometer]] |?Was submitted in year |?Has title |?Mammal and model=Organism |?Tissue and cell=Tissue;cell |format=broadtable |limit=5000 |offset=0 |sort=Was submitted in year |order=descending }} </div> </div> <br /> ==Additional resources== ::::» [[O2k-FluoRespirometer]] ::::» [[MiPNet22.11 O2k-FluoRespirometer manual]] ::::» [[MiPNet17.05 O2k-Fluo LED2-Module|O2k-Fluo Manual]]<br> ::::» [[O2k-Demo_experiments#O2k-Demo_O2k-MultiSensor |O2k-Demo experiments]]<br> ::::» [[O2k-Catalogue:_O2k-Modules|O2k-Modules]]<br> ::::» [[O2k-Innovation description|O2k-Innovation]]<br> [[File:Questions.jpg|left|40px]] <br /> <div class="toccolours mw-collapsible mw-collapsed"> ::: <span style="font-size:105%; color:##424242">'''» Keywords'''</span> <div class="mw-collapsible-content"> ::: '''Specific''' ::::» [[O2k-Fluorometer Series G]] ::::» [[Amplex UltraRed|Amplex® UltraRed]] ::::» [[Calcium green|Calcium Green™]] ::::» [[Magnesium green|Magnesium Green™]] ::::» [[Safranin]] ::::» [[TMRM]] ::::» [[Fluorescence-Control Unit]] ::::» [[Fluorescence-Sensor Blue]] ::::» [[Fluorescence-Sensor Green]] ::: '''General''' ::::» [[HRR]] ::::» [[mt-membrane potential]] ::::» [[O2k-chamber]] ::::» [[O2k-Core]] ::::» [[O2k-Main Unit]] ::::» [[O2k signals and output#O2k output |O2k output]] ::::» [[O2k signals and output#Signal of the O2k-Core and add-on modules |O2k signal]] ::::» [[Oroboros O2k]] </div> </div> <br />

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