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Difference between revisions of "O2k-Innovation"

From Bioblast
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== Tyrolean Government grant ==
== Tyrolean Government grant ==
[[File:Sponsorlogo gefoerdert RGB.jpg|right|350px]]
[[File:Sponsorlogo gefoerdert RGB.jpg|right|350px]]
* The project '''NextGen-O2k''' is supported by the "'''Technologieförderungsprogramm - Tiroler Innovationsförderung'''" of the Tyrolean Government.
::::* The project '''NextGen-O2k''' is supported by the "'''Technologieförderungsprogramm - Tiroler Innovationsförderung'''" of the Tyrolean Government.
* '''Project duration''': 2015-02-01 to 2017-01-31.
::::* '''Project duration''': 2015-02-01 to 2017-01-31.
* '''Partners''': [[OROBOROS:About| OROBOROS INSTRUMENTS]], [[OROBOROS partners| WGT Elektronik]], [[OROBOROS partners| software security networks]].
::::* '''Partners''': [[About Oroboros]], [[Oroboros partners| WGT Elektronik]], [[Oroboros partners| software security networks]].
* '''Application''': 2015-01-30; '''Granted''': 2015-03-24 (total budget € 350.000).
::::* '''Application''': 2015-01-30; '''Granted''': 2015-03-24 (total budget € 350.000).
* Funding: '''€ 140,000''' plus a bonus of '''€ 5,000''' for the implementation of gender sensitive actions.
::::* Funding: '''€ 140,000''' plus a bonus of '''€ 5,000''' for the implementation of gender sensitive actions.




== NextGen-O2k publication ==
== NextGen-O2k publication ==


* Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. - [[Harrison_2015_J_Appl_Physiol |»Bioblast link«]]
::::* Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. - [[Harrison_2015_J_Appl_Physiol |»Bioblast link«]]




== The fourth dimension of respiratory control ==
== The fourth dimension of respiratory control ==


# On the "line": one dimension is insufficient.
::::# On the "line": one dimension is insufficient.
# Substrate and coupling control are two necessarily connected dimensions defining the “area” of respiratory control.
::::# Coupling and pathway control are two necessarily connected dimensions defining the “area” of respiratory control.
# Adding kinetics (including substrate kinetics of O<sub>2</sub>, CHO, adenylates, P<sub>i</sub>, and flux-force kinetics) is the third dimension bringing “volume” into the picture of classical mitochondrial respiratory control.
::::# Adding kinetics (including substrate kinetics of O<sub>2</sub>, CHO, adenylates, P<sub>i</sub>, and flux-force kinetics) is the third dimension bringing “volume” into the picture of classical mitochondrial respiratory control.
# The NextGen-O2k on top of O2k-MultiSensor modules and the [[O2k-Fluorometer]] represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca<sup>2+</sup>, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O<sub>2</sub> and O<sub>2</sub>-flux in the same chamber.
::::# The NextGen-O2k on top of O2k-MultiSensor modules and the [[O2k-FluoRespirometer]] represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca<sup>2+</sup>, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O<sub>2</sub> and O<sub>2</sub>-flux in the same chamber.




== Development of the NextGen-O2k: a look inside ==
== Development of the NextGen-O2k: a look inside ==


::: Different stages of prototype development: The [[NextGen-O2k]] is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics.
:::: Different stages of prototype development: The [[NextGen-O2k]] is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics.


<gallery mode=default perrow=2 widths="500px" heights="300px">
<gallery mode=default perrow=2 widths="500px" heights="300px">

Revision as of 18:12, 15 July 2017

                



O2k-Innovation

A project in progress

Tyrolean Government grant

Sponsorlogo gefoerdert RGB.jpg
  • The project NextGen-O2k is supported by the "Technologieförderungsprogramm - Tiroler Innovationsförderung" of the Tyrolean Government.
  • Project duration: 2015-02-01 to 2017-01-31.
  • Partners: About Oroboros, WGT Elektronik, software security networks.
  • Application: 2015-01-30; Granted: 2015-03-24 (total budget € 350.000).
  • Funding: € 140,000 plus a bonus of € 5,000 for the implementation of gender sensitive actions.


NextGen-O2k publication

  • Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. - »Bioblast link«


The fourth dimension of respiratory control

  1. On the "line": one dimension is insufficient.
  2. Coupling and pathway control are two necessarily connected dimensions defining the “area” of respiratory control.
  3. Adding kinetics (including substrate kinetics of O2, CHO, adenylates, Pi, and flux-force kinetics) is the third dimension bringing “volume” into the picture of classical mitochondrial respiratory control.
  4. The NextGen-O2k on top of O2k-MultiSensor modules and the O2k-FluoRespirometer represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca2+, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O2 and O2-flux in the same chamber.


Development of the NextGen-O2k: a look inside

Different stages of prototype development: The NextGen-O2k is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics.