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O2k-Innovation

From Bioblast
Revision as of 13:59, 16 August 2015 by Gnaiger Erich (talk | contribs)

                



O2k-Innovation

A project in progress

Tyrolean Government grant

Sponsorlogo gefoerdert RGB.jpg
  • The project NextGen-O2k is supported by the "Technologieförderungsprogramm - Tiroler Innovationsförderung" of the Tyrolean Government.
  • Project duration: 2015-02-01 to 2017-01-31.
  • Partners: OROBOROS INSTRUMENTS, WGT Elektronik, software security networks.
  • Application: 2015-01-30; Granted: 2015-03-24 (total budget € 350.000).
  • Funding: € 140,000 plus a bonus of € 5,000 for the implementation of gender sensitive actions.


NextGen-O2k publication

  • Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol Aug 6:jap.00146.2015. doi: 10.1152/japplphysiol.00146.2015. [Epub ahead of print]. - »Bioblast link«


The fourth dimension of respiratory control

  1. On the "line": one dimension is insufficient.
  2. Substrate and coupling control are two necessarily connected dimensions defining the “area” of respiratory control.
  3. Adding kinetics (including substrate kinetics of O2, CHO, adenylates, Pi, and flux-force kinetics) is the third dimension bringing “volume” into the picture of classical mitochondrial respiratory control.
  4. The NextGen-O2k on top of O2k-MultiSensor modules and the O2k-Fluorometer represent the fourth dimension of respiratory control, by adding analysis of functional variables (such as ROS production, mt-membrane potential, Ca2+, proton flux, cytochrome and NADH redox states, ATP production) to the measurement of O2 and O2-flux in the same chamber.


Development of the NextGen-O2k: a look inside

Different stages of prototype development: The NextGen-O2k is becoming more and more packed with two spectrofluorometers, two spectrophotometers, light guides, Peltier temperatue control with new heat pipes, microelectronics.