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Difference between revisions of "O2k-Open Support"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
 
[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
::::<big>'''- we provide service and support based on open innovation'''</big>       
<big>'''We provide service and support » [[O2k-Open Support alert]]''' </big>       
 
<br />
[[File:O2k-Support.jpg|left|200px|Oroboros support]]


__TOC__
__TOC__


[[File:MitoPedia.jpg|left|100px|MitoPedia|link=MitoPedia]]
== O2k-Open Support: technical help ==


== MitoPedia ==
:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
::::::## '''[[MitoPedia: O2k-Open Support]]'''
::::::## [[MitoPedia: O2k hardware]]
::::::## [[MitoPedia: DatLab]]
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
::::::# [[O2k-Videosupport]]
::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].


:::: Find information quickly with '''[[MitoPedia|MitoPedia]]''', the Bioblast glossary with lists of key words and links to relevant pages, specifically and the O2k and high-resolution respirometry:     
<br/>
::::» [[MitoPedia:_O2k_hardware]]
::::» [[MitoPedia:_DatLab]]
::::» [[MitoPedia:_SUIT]]: Substrate-uncoupler-inhibitor-titration protocols     
[[File:O2k-Manual.jpg|left|100px|O2k-Manual|link=http://wiki.oroboros.at/index.php/O2k-Manual]]
== O2k-Manual ==
::::The '''[[O2k-Manual|O2k-Manual]]''' helps beginners and experienced users. Context sensitive help is provided in the DatLab software: '''» [[MitoPedia:_DatLab]]'''     
::::» Polarographic oxygen sensors: calibration, accuracy and quality control SOP » [[MiPNet06.03_POS-calibration-SOP|MiPNet06.03 POS-calibration-SOP]]
::::» Instrumental O<sub>2</sub> background correction, accuracy of oxygen flux and SOP » [[MiPNet14.06_Instrumental_O2_background|MiPNet14.06 Instrumental O2 background]]     
<br/>
[[File:PlayVideo.jpg|left|100px|O2k-Videosupport|link=http://wiki.oroboros.at/index.php/O2k-Videosupport]]
== O2k-Videosupport ==
::::» '''[[O2k-Videosupport|O2k-Videosupport]]'''     
[[File:O2k-Protocols.jpg|left|100px|O2k-Procedures|link=http://wiki.oroboros.at/index.php/O2k-Procedures]]
== O2k-Procedures ==
::::'''O2k-Procedures''' explain various applications of the O2k with O2k-Demo experiments, and more: '''» [[O2k-Procedures]]'''     
{| class="wikitable" border="1"
|-
| [[File:O2k-Protocols.jpg|left|60px|O2k-Procedures]]
| <big>» [[O2k-Procedures:_MitoPathways]]</big>
|-
| [[File:Pesta 2012 Methods Mol Biol Fig7A.jpg|left|60px|Pesta 2012 Methods Mol Biol Fig7A.jpg|link=Pesta 2012 Methods Mol Biol/SUIT-Protocol]]
| <big>» [[O2k-Demo_experiments|O2k-Demo experiments]]</big>
|-
| [[File:O2k-Protocols.jpg|left|60px|O2k-Procedures]]
| <big>» [[O2k-Chemicals_and_media|O2k-Chemicals and media]]</big>
|-
| [[File:PBI-Shredder HRR-Set.JPG|left|30px|PBI-Shredder O2k-Set|link=PBI-Shredder O2k-Set]]
| <big>» [[O2k-mitochondrial_preparations|O2k-mitochondrial preparations]]</big>
|-
| [[File:O2k-Protocols.jpg|left|60px|O2k-Procedures]]
| <big>» [[O2k-Procedures:_Mitochondrial_and_marker-enzymes]]</big>
|}
::::* Implement your quality control management with the [[MitoFit_Quality_Control_System|MitoFit QCS]], to diagnose technical problems fast and cost-effectively with instrumental quality control tests. 
[[File:Questions.jpg|left|50px|Questions.jpg]]
== Frequently Asked Questions ==
::::» '''[[Oroboros_FAQ|Oroboros FAQ]]'''     
[[File:O2k-support system.jpg|right|300px|Oroboros support system]]
== Open innovation ==
::::* '''Open innovation''' extends the support given to individual users to the scientific community and provides feedback to improve the scope and documentation of the O2k-technology - in the spirit of [[Gentle_Science|Gentle Science]].
::::* We welcome and value feedback. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, will be relevant to both specialists and beginners in the field.
:::<big>'''Extended O2k-technical support'''</big>
::::* O2k-technical support is defined as '''extended''' if support is requested on O2k-technology and high-resolution respirometry without prior consulting the [[O2k-Manual|O2k-Manual]] and relevant chapters on [[O2k-Procedures]].
::::* Regular consultancy fees apply for '''extended O2k-technical support''' requested without open innovation agreement.
::::* Acceptance of '''extended O2k-technical support''' with an '''open innovation agreement''' implies that Oroboros Instruments may publish the relevant correspondence or a summary of the correspondence addressing technical and scientific questions on our website, including names, institutional and Email addresses.       


::: '''<big>O2k-Quality Control</big>'''
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].     


[[File:O2k-Support.jpg|left|100px|Oroboros support]]
:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
 
::::# Clean O2k-chambers and contamination-free incubation medium are required.  
== O2k-technical support ==
::::# Analysis of DatLab files recorded in instrumental QC tests is essential.       
 
::: '''Application'''
:::: An '''application problem''' can be diagnosed on the basis of the O2 sensor and O2k-chamber tests. If proper instrumental performance is obtained, software and hardware problems are excluded.     
 
::: '''Software'''
:::: Service for DatLab 6 and previous versions is discontinued.     
 
::: '''Hardware'''
 
::::# Hardware problems may be solved by user-service: In particular, the [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires user-service at intervals (these may be more than 1 year), and SOPs are available to determine if a sensor service is required at an earlier or later date.
::::# Hardware problems may require replacement of a defective spare part.
::::# Some electronic or mechanical defects may be solved only by service of the O2k in the workshop of Oroboros Instruments, e.g. a defective Peltier unit.       
 
[[File:O2k-QCS.jpg|right|400px|O2k-QCS]]
 
=== O2k Quality Control ===
 
:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (Instrumental quality control: O2k-QC), distinguished from Assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols, and SUIT protocols.  
::::# Biological experiments are not suitable for trouble shooting.  
::::# Biological experiments are not suitable for trouble shooting.  
::::# Clean O2k-Chambers and contamination-free incubation medium are required.
::::# Analysis of DatLab files recorded in such instrumental tests is essential.       


:::: The '''sequence''' of Instrumental QC tests is strictly  
:::: The '''sequence''' of instrumental QC tests is strictly  
::::# O2 sensor test,
::::# O2k-chamber test.       


[[File:O2 sensor test.jpg|right|400px|O2 sensor test|link=MiPNet06.03 POS-calibration-SOP]]
=== 1. O2 sensor test ===
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
::::» O2 sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]      


==== O2 sensor test ====
=== 2. O2k-chamber test ===
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background''']]     


:::: Many technical problems with the [[Gain_(O2_channel)|O2 channel]] can be analyzed and solved with the '''O2 sensor test''', described in detail:  
[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
::::» O2 sensor test: [[MiPNet06.03_POS-calibration-SOP|'''QC1 POS-calibration''']]      
== O2k-Open Support agreement ==


 
:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
==== O2k-chamber test ====
::::» [[Search for defective O2k components]]
 
:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [mailto:support@oroboros.at| support@oroboros.at]        
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-Chamber assembly has to be tested, described in detail:
::::» [[O2k-Open Support agreement]]
::::» O2k-chamber test: [[MiPNet14.06_Instrumental_O2_background|'''QC2 Instrumental O<sub>2</sub> background''']]      
::::» [[O2k repair]]
 
 
=== The 2-chamber design of the O2k ===
 
:::: The '''two chamber design''' of the O'''2k''' has many benefits, including advantages in '''trouble shooting'''. By switching components between sides A and B of the O2k, it is usually possible to locate an instrumental problem, finding selectively a specific defective component of the O2k. This component can then be serviced by the end user or replaced by Oroboros without the need to ship the entire O2k.       
 
 
=== Cost estimation for repair ===
 
:::: A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k by our workshop in Austria. Once a cost estimation has been provided, the repair is carried out after the customer’s consent. For shipment of the O2k, follow the [[Shipping_an_oxygraph|detailed instructions]].     




[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
== The O2k-Network ==
== The O2k-Network ==


:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.       
:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.       


:::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]       
::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]       
 
:::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]     
 
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
 
== Contact ==


:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [mailto:support@oroboros.at| support@oroboros.at]       
::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]       


:::: '''»''' [[Oroboros_MitoFit_Laboratory|Oroboros MitoFit Laboratory]] - open for innovation and cooperation.  
:::: '''»''' [[Oroboros Laboratories|Oroboros O2k-Laboratory]] - open for innovation and cooperation.  
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       


[[Category:OroboPedia]]
[[Category:O2k-Open Support]]

Revision as of 09:40, 13 February 2020


                  


O2k-Open Support

O2k-Open Support


Oroboros support system

We provide service and support » O2k-Open Support alert

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
  1. MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
    1. MitoPedia: O2k-Open Support
    2. MitoPedia: O2k hardware
    3. MitoPedia: DatLab
  2. O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
  3. O2k-Manual: topic specific manuals with step by step help
  4. O2k-Videosupport
  5. O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.


O2k-Quality Control
MitoFit-QCS
Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.
The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
  1. Clean O2k-chambers and contamination-free incubation medium are required.
  2. Analysis of DatLab files recorded in instrumental QC tests is essential.
  3. Biological experiments are not suitable for trouble shooting.
The sequence of instrumental QC tests is strictly

1. O2 sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
» O2 sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
» O2k-chamber test: QC2: Instrumental O2 background
O2k-Open Support

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.
» Search for defective O2k components
» In case you need further technical support and for your valuable feedback, please contact support@oroboros.at
» O2k-Open Support agreement
» O2k repair


O2k-Network

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
» The O2k-Network Labs: » O2k-Network
» O2k-Network discussion forum
» Oroboros O2k-Laboratory - open for innovation and cooperation.
» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry