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Difference between revisions of "O2k-Open Support"

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{{Template:OROBOROS support page name}}
{{Template:OROBOROS support page name}}
[[File:O2k-support system.jpg|right|350px|Oroboros support system]]
:::::::::::: <big>''Currently Asked Questions - »'''[[O2k-Open Support alert]]'''« </big>
__TOC__


::::<big>'''- we provide service and support based on open innovation'''</big>     
== O2k-Open Support: technical help ==
 
[[File:O2k-Support.jpg|left|200px|Oroboros support]]


__TOC__
:::: '''O2k-Open Support''' includes [[Open Access]] pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
::::::# '''MitoPedia: O2k''': find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
::::::## '''[[MitoPedia: O2k-Open Support]]'''
::::::## [[MitoPedia: O2k hardware]]
::::::## [[MitoPedia: DatLab]]
::::::# [[O2k-Procedures]]: explain various applications of the O2k with O2k-Demo experiments
::::::# [[O2k-Manual]]: topic specific manuals with step by step help
::::::# [[O2k-Videosupport]]
::::::# [[O2k-Products]]: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of [[Gentle Science| Gentle Science and Open Science]].


[[File:O2k-QCS.jpg|right|400px|O2k-QCS]]
=== O2k Quality Control ===


::: '''<big>O2k-Quality Control</big>'''
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].       
:::: Many commonly raised issues of O2k-technical support are solved in the newest version of [[DatLab]].       


:::: Hardware problems may be solved by technical service procedures carried out regularly by the user: In particular, the [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date.
:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].
 
::::# Clean O2k-chambers and contamination-free incubation medium are required.
:::: The '''O2 sensor and O2k chamber tests''' are important to eliminate instrumental artefacts (Instrumental quality control: O2k-QC), distinguished from Assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols, and SUIT protocols.  
::::# Analysis of DatLab files recorded in instrumental QC tests is essential.        
::::# Biological experiments are not suitable for trouble shooting.  
::::# Biological experiments are not suitable for trouble shooting.  
::::# Clean O2k-Chambers and contamination-free incubation medium are required.
::::# Analysis of DatLab files recorded in such instrumental tests is essential.       
:::: The '''sequence''' of Instrumental QC tests is strictly
::::# O2 sensor test,
::::# O2k-chamber test.       


[[File:O2 sensor test.jpg|right|400px|O2 sensor test|link=MiPNet06.03 POS-calibration-SOP]]
:::: The '''sequence''' of instrumental QC tests is strictly


==== O2 sensor test ====
=== 1. O2 sensor test ===
:::: The [[OroboPOS|OroboPOS]] (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
::::» O2 sensor test: [[Oxygen_sensor_test |'''QC1: Oxygen sensor test''']]     


:::: Many technical problems with the [[Gain_(O2_channel)|O2 channel]] can be analyzed and solved with the '''O2 sensor test''', described in detail:  
=== 2. O2k-chamber test ===
::::» O2 sensor test: [[Oxygen_sensor_test|'''Oxygen sensor test''']]       
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:  
::::» O2k-chamber test: [[Oxygen flux - instrumental background |'''QC2: Instrumental O<sub>2</sub> background''']]       


[[File:O2k-Support.jpg|right|200px|O2k-Open Support]]
== O2k-Open Support agreement ==


==== O2k-chamber test ====
:::: The Oroboros experts will be happy to help in using our O2k-Open Support system.
 
::::» [[Search for defective O2k components]]
:::: If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-Chamber assembly has to be tested, described in detail:
:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [https://www.oroboros.at/index.php/o2k-technical-support '''O2k-Tech Support''']        
::::» O2k-chamber test: [[MiPNet14.06_Instrumental_O2_background|'''QC2 Instrumental O<sub>2</sub> background''']]      
::::» [[O2k-Open Support agreement]]
 
::::» [[O2k repair]]
 
=== The 2-chamber design of the O2k ===
 
:::: The '''two chamber design''' of the O'''2k''' has many benefits, including advantages in '''trouble shooting'''. By switching components between sides A and B of the O2k, it is usually possible to locate an instrumental problem, finding selectively a specific defective component of the O2k. This component can then be serviced by the end user or replaced by Oroboros without the need to ship the entire O2k.        
 
 
=== Beyond regular service by the user ===
 
:::: Hardware problems may require replacement of a defective spare part. Some electronic or mechanical defects may be solved only by service of the O2k in the workshop of Oroboros Instruments, e.g. a defective Peltier unit.       
 
=== Cost estimation for repair ===
 
:::: A cost estimation for repair of the O2k-Main Unit due to an electronic or mechanical defect can only be given after inspection of the O2k by our workshop in Austria. Once a cost estimation has been provided, the repair is carried out after the customer’s consent. For shipment of the O2k, follow the [[Shipping_an_oxygraph|detailed instructions]].     
 
[[File:O2k-support system.jpg|right|300px|Oroboros support system]]




[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
[[File:O2k-Network.png|left|100px|O2k-Network|link=O2k-Network]]
== The O2k-Network ==
== The O2k-Network ==


:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.       
:::: The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.       


:::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]       
::::: '''»''' The O2k-Network Labs: [[O2k-Network|» O2k-Network]]       
 
:::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]     
 
[[File:MitoFit-QCS.jpg|right|400px|MitoFit-QCS]]
 
 
== Open Science ==
 
::::* '''Open Science''' extends the support given to individual users to the scientific community and provides feedback to improve the scope and documentation of the O2k-technology - in the spirit of [[Gentle_Science|Gentle Science]].
::::* We welcome and value feedback. Technical and scientific questions, which are not yet addressed in the available guidelines or are difficult to trace on our website, will be relevant to both specialists and beginners in the field.
 
:::<big>'''Open O2k-technical support'''</big>


::::* O2k-technical support does not replace the obligation prior consulting the [[O2k-Manual|O2k-Manual]] and relevant chapters on [[O2k-Procedures]].
::::: '''»''' [[O2k-Network_discussion_forum|O2k-Network discussion forum]]      
::::* '''Open O2k-technical support''' requires an '''open support agreement''' for each individual case. Oroboros Instruments may then publish the relevant correspondence or a summary of the correspondence addressing technical and scientific questions on our website. Our users can decide if their names, institutional and Email addresses are included.       
::::* Regular consultancy fees apply for '''O2k-technical support''' requested without open support agreement.


== Contact ==
:::: '''»''' [[Oroboros Laboratories|Oroboros O2k-Laboratory]] - open for innovation and cooperation.  
 
:::: '''»''' In case you need further technical support and for your valuable feedback, please contact [mailto:support@oroboros.at| support@oroboros.at]     
 
:::: '''»''' [[Oroboros_MitoFit_Laboratory|Oroboros MitoFit Laboratory]] - open for innovation and cooperation.  
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       
:::: '''»''' [[Oroboros_Science_Scholarship|Oroboros Science Scholarships]] - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry       


 
[[Category:O2k-Open Support]]
== Search keywords for technical support ==
::::» [[:Category:Keywords |Alphabetical list of keywords]]
 
[[Category:OroboPedia]]

Revision as of 07:46, 19 March 2020


                  


O2k-Open Support

O2k-Open Support


Oroboros support system
Currently Asked Questions - »O2k-Open Support alert« 

O2k-Open Support: technical help

O2k-Open Support includes Open Access pages that help to optimally use the O2k, to apply instrumental O2k-Quality Control, and perform user-service when needed.
  1. MitoPedia: O2k: find information quickly with MitoPedia, the Bioblast glossary with lists of key words and links to relevant pages.
    1. MitoPedia: O2k-Open Support
    2. MitoPedia: O2k hardware
    3. MitoPedia: DatLab
  2. O2k-Procedures: explain various applications of the O2k with O2k-Demo experiments
  3. O2k-Manual: topic specific manuals with step by step help
  4. O2k-Videosupport
  5. O2k-Products: is not merely a 'List of products', it presents a guided tour to the O2k and integrates the concept of Gentle Science and Open Science.


O2k-Quality Control
MitoFit-QCS
Many commonly raised issues of O2k-technical support are solved in the newest version of DatLab.
The O2 sensor and O2k chamber tests are important to eliminate instrumental artefacts (instrumental quality control, QC), distinguished from assay QC addressing problems of solution protocols (preparation of chemicals), sample preparation protocols (such as mitochondrial preparations), and SUIT protocols.
  1. Clean O2k-chambers and contamination-free incubation medium are required.
  2. Analysis of DatLab files recorded in instrumental QC tests is essential.
  3. Biological experiments are not suitable for trouble shooting.
The sequence of instrumental QC tests is strictly

1. O2 sensor test

The OroboPOS (polarographic oxygen sensor) requires regular service at intervals - these may be more than 1 year. SOPs are available to determine if a sensor service is required at an earlier or later date:
» O2 sensor test: QC1: Oxygen sensor test

2. O2k-chamber test

If all quality control criteria of the O2 sensor test are met, the operator can be assured that the quality of the sensor signal is acceptable. Next, the quality of the O2k-chamber assembly has to be tested, described in detail:
» O2k-chamber test: QC2: Instrumental O2 background
O2k-Open Support

O2k-Open Support agreement

The Oroboros experts will be happy to help in using our O2k-Open Support system.
» Search for defective O2k components
» In case you need further technical support and for your valuable feedback, please contact O2k-Tech Support
» O2k-Open Support agreement
» O2k repair


O2k-Network

The O2k-Network

The Oroboros O2k-Network serves to connect and support: Contact for advice on applications of the O2k and high-resolution respirometry - the global network in mitochondrial physiology.
» The O2k-Network Labs: » O2k-Network
» O2k-Network discussion forum
» Oroboros O2k-Laboratory - open for innovation and cooperation.
» Oroboros Science Scholarships - are offered for focused research projects on mitochondrial physiology and high-resolution respirometry