Difference between revisions of "O2k-Open Support alert"
From Bioblast
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__TOC__ | __TOC__ | ||
== Feb 2020 == | |||
* '''Feb-29 Sa''' | |||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files''' | |||
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]« | |||
== Jan 2020 == | == Jan 2020 == | ||
*'''Jan-31 Fr''' | * '''Jan-31 Fr''' | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration''' | ||
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). | ::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »[[Oxygen calibration - DatLab]]« | ||
*'''Jan-30 Th''' | * '''Jan-30 Th''' | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?''' | ||
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. | ::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »[[Oxygen calibration - DatLab]]« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ‘R1’ and complete the air calibration correctly?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ‘R1’ and complete the air calibration correctly?''' | ||
::::::: Your air calibration is not effective until you set mark ‘R1’ andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. | ::::::: Your air calibration is not effective until you set mark ‘R1’ andclick on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - »[[Oxygen calibration - DatLab]]« | ||
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:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?''' | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?''' | ||
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). »[[O2k-Chamber MitoPedia]]« | ::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]« | ||
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab? | :::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab? | ||
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. »[[Oxygen solubility factor]]« | ::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]« | ||
== Stay alert == | == Stay alert == | ||
{{Oroboros Ecosystem Agenda list}} | {{Oroboros Ecosystem Agenda list}} |
Revision as of 11:52, 29 February 2020
O2k-Open Support alert |
We provide service and support based on open innovation
- - listed in the Oroboros Ecosystem Agenda 2020
Feb 2020
- Feb-29 Sa
- Calibration parameters are transferred to experimental DatLab files
- Save calibration information from selected Marks while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - » Oxygen calibration - DatLab«
- Calibration parameters are transferred to experimental DatLab files
Jan 2020
- Jan-31 Fr
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »Oxygen calibration - DatLab«
- Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration
- Jan-30 Th
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O2 slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »Oxygen calibration - DatLab«
- Do you check the O2 flux at the end of the air calibration with the O2k-chamber closed?
- Your air calibration is not effective until you set mark ‘R1’ andclick on Calibrate and copy to clipboard in the O2 calibration window. - »Oxygen calibration - DatLab«
- Jan-24 Fr
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. »O2k configuration«
- Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?
- Do you perform O2k-Chamber volume calibration?
- The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the O2k-sV-Module). - »O2k-Chamber MitoPedia«
- Do you perform O2k-Chamber volume calibration?
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
- The O2 solubility factor varies according to the medium used and influences the air calibration. - »Oxygen solubility factor«
- Do you use the correct O2 solubility factor (FM) in the ‘O2 calibration’ window in DatLab?
Stay alert
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