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Difference between revisions of "O2k-Open Support alert"

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{{Template:OROBOROS support page name}}
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[[File:O2k-support system.jpg|right|350px|Oroboros support system|link=https://wiki.oroboros.at/index.php/O2k-Open_Support]]
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:::::: <big>''Currently Answered Questions, '''CAQ''''', from the »[[Oroboros Ecosystem Agenda 2020 |Oroboros Ecosystem Agenda]]«</big>
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{| role="presentation" id="mp-upper" style="width: 100%; margin-top:4px; border-spacing: 0px;"
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<div id="mp-tfa-h2" style="margin:0.5em; background:#2856ba; font-family:inherit; font-size:120%; font-weight:bold; border:1px solid #a3bfb1; color:#ffffff; padding:0.2em 0.4em;">O2k-Open Support alert - 2021</div>
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<div id="mp-itn" style="padding:1em 1em;">[[File:O2k-support system.jpg|center|350px|Oroboros Ecosystem Agenda ]]</div>
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<div style="margin:0.5em; background:#cedff2; font-family:inherit; font-size:120%; font-weight:bold; border:1px solid #a3b0bf; color:#000; padding:0.2em 0.4em;">O2k Support Alert Legend</div>
<div id="mp-itn" style="padding:0.1em 0.6em;">[[File:Footer O2k-OpenSupport-Alert.jpg|center|500px|Oroboros Ecosystem Agenda ]]</div>
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<!--        STAY ALERT        -->


== Mar 2020 ==
<div style="margin:0.5em; background:#cedff2; font-family:inherit; font-size:120%; font-weight:bold; border:1px solid #a3b0bf; color:#000; padding:0.2em 0.4em;">Stay alert</div>
<div id="mp-itn" style="padding:0.1em 0.6em;">{{Oroboros Ecosystem Agenda list}}</div>
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» [[O2k-OpenSupportAlert2020 Archive]]
* '''Mar-04 We'''
[[File:OroboPOS-Holder002.jpg|right|100px]]
<br />
 
::'''''New''''' [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.'''
::::::: See how you can check that the two blue OroboPOS-Holders are intact. - »[[O2k-OroboPOS-Holder_MitoPedia#O2k-OroboPOS-Holder:_Leaky_chamber_due_to_damaged_OroboPOS-Holder |O2k-OroboPOS-Holder]]«
<br />
 
 
 
== Feb 2020 ==
* '''Feb-28 Fr'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Calibration parameters are transferred to experimental DatLab files'''
::::::: Save calibration information from selected [[Marks - DatLab |Marks]] while DatLab is connected to the O2k during an instrumental calibration run. Air calibration and zero oxygen calibration can be performed and saved in a single DatLab file or in separate DatLab files. - »[[Oxygen_calibration_-_DatLab#Oxygen_calibration_in_instrumental_and_experimental_DatLab_files | Oxygen calibration - DatLab]]«
 
== Jan 2020 ==
 
* '''Jan-31 Fr'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]][[File:Yellow-take care.png|40px|Take care]] '''Reproducibility committment in high-resolution respirometry: quality control in oxygen calibration'''
::::::: In the DatLab 7.4 software, new instrumental DL-Protocols guide the user automatically step-by-step, to calibrate the instrument accurately. Reproducibility is monitored and documented when following user-friendly standard operating procedures (SOP). - »[[Oxygen calibration - DatLab]]«
 
 
* '''Jan-30 Th'''
 
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you check the O<sub>2</sub> flux at the end of the air calibration with the O2k-chamber closed?'''
::::::: In DatLab 7.4, instrumental DL-Protocols guide you automatically towards closing the O2k-chamber after air calibration, to monitor "O<sub>2</sub> slope neg." (negative) as a test of any biological contamination which would increase oxygen consumption in the absence of a biological sample. - »[[Oxygen calibration - DatLab]]«
 
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Blue-info.png|40px|Info: improve]] '''Do you set mark ‘R1’ and complete the air calibration correctly?'''
 
::::::: Your air calibration is not effective until you set mark ‘R1’ and click on <span style="background:lightgrey">Calibrate and copy to clipboard</span style> in the <span style="background:lightgrey">O2 calibration</span style> window. - »[[Oxygen calibration - DatLab]]«
 
 
*'''Jan-24 Fr'''
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]]  [[File:Blue-info.png|40px|Info: improve]] '''Do you write the serial number of the polarographic oxygen sensor (POS) in the ‘O2k configuration’ window?'''
::::::: Including the POS serial number in the ´O2k configuration´ window is important to keep a track record of the sensors used according to the O2k. This will be especially helpful if troubleshooting is needed. »[[O2k configuration]]«
 
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Red-loss of data.png|40px|Warning: loss of data]] '''Do you perform O2k-Chamber volume calibration?'''
::::::: The volume calibration is essential to ensure that the chamber volume is correctly calibrated to its standard volume of 2 mL (or 0.5 mL for the [[O2k-sV-Module]]). - »[[O2k-Chamber MitoPedia]]«
 
:::: [[Image:O2k-Support.jpg|60px|link=http://wiki.oroboros.at/index.php/O2k-Open_Support |O2k-Open Support]] [[File:Yellow-take care.png|40px|Take care]] '''Do you use the correct O<sub>2</sub> solubility factor (F<sub>M</sub>) in the ‘O2 calibration’ window in DatLab?
::::::: The O<sub>2</sub> solubility factor varies according to the medium used and influences the air calibration. - »[[Oxygen solubility factor]]«
 
 
== Stay alert ==
{{Oroboros Ecosystem Agenda list}}

Revision as of 15:00, 5 January 2021


                  


» O2k-OpenSupportAlert2020 Archive