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| | » [[O2k-OpenSupportAlert2020 Archive]] |
Revision as of 16:00, 5 January 2021
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O2k-Open Support alert - 2021
Dec 2021
 
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How do you measure ROUTINE respiration?
ROUTINE respiration (R) of living cells is measured before permeabilization of the cell membrane either in cell culture medium or any other respiration media (e.g. MiR05) in the presence or absence of exogenous substrates. Allow R to stabilize for 15-20 min.
- »ROUTINE respiration« and »Doerrier 2018 Methods Mol Biol«
- communicated by »Komlodi Timea« and »Schmitt Sabine«
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Nov 2021
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What is the difference between the O2k-Fluo Smart-Module and the O2k-Fluo LED2-Module?
The O2k-Fluo Smart-Module, compatible with O2k-series H-I, improves upon the O2k-Fluo LED2-Module for O2k-series D-G, with pre-calibrated Smart Fluo-Sensors that provide direct input into DatLab and have sensor-specific memory to obtain reproducible light intensities with different sensors.
- »O2k-Fluo Smart-Module and O2k-Fluo LED2-Module«
- communicated by »Komlodi Timea« and »Cecatto Cristiane «
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Oct 2021
Sep 2021
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Can I compare Amplex UltraRed (AmR) experiments in which the H2O2 sensitivity is different?
The H2O2 sensitivity is calculated to calibrate the raw fluorescence signal to H2O2 equivalent concentration. Using the same gain and fluorescence intensity it is possible to compare experiments with different sensitivities.
- »AmR assay H2O2 sensitivity«
- communicated by »Cardoso Luiza« and »Komlodi Timea«
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Aug 2021
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Is it normal to see a pink coloring in the medium at the end of an experiment with Amplex UltraRed (AmR)?
The pink coloring is due to the accumulation of UltroxRed, generated from Amplex UltraRed reacting with H2O2.
- »AmR assay and color of the medium«
- communicated by »Cardoso Luiza« and »Komlodi Timea«
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What is the advantage of using flux control ratios (FCRs)?
FCRs provide information on coupling and pathway control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) determination of tissue mass or mt-markers.
- »Flux control ratio«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«
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Jul 2021
Jun 2021
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Do I need to calibrate the fluorescence signal with safranin?
It is possible to run the experiment without calibration, presenting only the fluorescence intensity plot. However, a simple two-point or multiple-point calibration for safranin concentration is possible.
- »Safranin calibration«
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Simultaneous fluctuations in O2 slope neg. and the signal of the Peltier power?
This can indicate an electrical disturbance and changing the electrical supply may resolve this problem.
- »Troubleshooting«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«
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May 2021
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Do you enter the correct instrumental background correction in DatLab for the experimental O2 concentrations?
After performing the instrumental background test at the appropriate O2 range, load the parameters in DatLab to ensure accurate correction for external fluxes.
- »O2 background correction in DatLab
- communicated by »Komlodi Timea« and »Cecatto Cristiane«
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Apr 2021
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Do you know what to do when the fluorescence signal starts at a negative value?
A negative signal may be caused by wet Fluo-Sensors. For further information:
- »Negative fluorescence signal«
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A damaged OroboPOS-Holder may cause medium to leak from the O2k-Chamber.
See how you can check that the OroboPOS-Holders are intact.
- »O2k-OroboPOS-Holder«
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Mar 2021
Feb 2021
Jan 2021
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O2k Support Alert Legend
Stay alert
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» O2k-OpenSupportAlert2020 Archive
