Can I compare Amplex UltraRed (AmR) experiments in which the H2O2 sensitivity is different?
The H2O2 sensitivity is calculated to calibrate the raw fluorescence signal to H2O2 equivalent concentration. Using the same gain and fluorescence intensity it is possible to compare experiments with different sensitivities.
- »AmR assay H2O2 sensitivity«
- communicated by »Cardoso Luiza« and »Komlodi Timea«
Is it normal to see a pink coloring in the medium at the end of an experiment with Amplex UltraRed (AmR)?
What is the advantage of using flux control ratios (FCRs)?
FCRs provide information on coupling and pathway control of mitochondrial respiration independent of (i) mt-content in cells or tissues, (ii) purification of isolated mitochondrial preparations, and (iii) determination of tissue mass or mt-markers.
- »Flux control ratio«
- communicated by »Cardoso Luiza« and »Cecatto Cristiane«
Do you know how to export a user-specific DL-protocol (*.DLPU)?
Do I need to calibrate the fluorescence signal with safranin?
It is possible to run the experiment without calibration, presenting only the fluorescence intensity plot. However, a simple two-point or multiple-point calibration for safranin concentration is possible.
- »Safranin calibration«
Simultaneous fluctuations in O2 slope neg. and the signal of the Peltier power?