O2k-Open Support alert - 2023
Dec 2023
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Do you observe a high signal at zero oxygen?
If the quality of your dithionite solution has already been checked, perform a leak current test to evaluate which electrical component is generating the out-of-scale signal.
- »Leak current test«
- communicated by »Baglivo Eleonora« and »Grings Mateus«
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- Dec- 11 Mo - International Mountain Day
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Mountain slopes can be difficult, but O2 slopes can be smooth with DatLab
The default setting of Slope smoothing is 40 data points, corresponding to an interval of 80 s at a data recording interval of 2 s. DatLab 7 expresses it in data points and DatLab 8 in seconds.
- »Smoothing in Flux/Slope«
- communicated by »Timon-Gomez Alba« and »Baglivo Eleonora«
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Is DatLab 8 compatible with your operative system?
The DatLab 8 software is designed for 64-bit versions of Windows operating systems and does not run on MAC devices.
- »DatLab 8«
- communicated by »Leo Elettra« and »Baglivo Eleonora«
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Nov 2023
Oct 2023
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Noticing higher values of J°1 when using the O2k-Chamber sV (0.5 mL) compared to the 2 mL O2k-Chamber?
The instrumental oxygen background flux, J°O2, is volume-dependent in the closed chamber; therefore, the values of J°1 are about four times higher in the sV chamber.
- »O2k-sV-Module«, »POS calibration-SOP«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«
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Sep 2023
Aug 2023
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Can I compare data sets recorded with different sensor gain settings?
Oxygen signals obtained after proper calibration are independent of the gain settings, therefore oxygen concentrations (and fluxes) can be compared, as long as the corresponding air and zero calibrations are applied.
- »Gain of the O2 channel«
- communicated by »Baglivo Eleonora« and »Leo Elettra«
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Jul 2023
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Are you planning to perform several measurements in one day using the same O2k-chamber?
To avoid any possible contamination by inhibitors in the subsequent measurements, the O2k-chamber needs to be cleaned each time with the standard protocol AfterUse or AfterUse_inhibitors.
- »O2k-cleaning - Sections 2.3 and 2.4«
- communicated by »Timon-Gomez Alba« and »Cecatto Cristiane«
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May 2023
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Does your polarographic oxygen sensor (POS) need a deep clean?
A POS with a very dark anode might need several rounds of ammonia cleaning, possibly including prolonged exposure overnight to restore performance specifications.
- »POS service«
- communicated by »Cecatto Cristiane« and »Leo Elettra«
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Mar 2023
Feb 2023
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Add catalase when measuring Complex IV (CIV) activity in permeabilized fibers (pfi)
Pfi have a high content of iron-containing proteins, which accelerate the autoxidation of tetramethyl-p-phenylenediamine (TMPD) in the CIV assay. Catalase prevents the accumulation of H2O2 generated by this autoxidation.
- »Chemical Background« and »Complex IV«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«
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Do you observe simultaneous fluctuations in the O2 slope neg. and the Peltier power?
This can indicate fluctuations in the room temperature or electrical supply. Controlling the temperature or changing the electrical supply may resolve this problem.
- »Troubleshooting«
- communicated by »Cecatto Cristiane« and »Schmitt Sabine«
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