O2k-Open Support alert

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O2k-Open Support alert - 2024

Currently Answered Questions from the »Oroboros Ecosystem Agenda«

Apr 2024

Apr-18 Th

How to store the POS overnight and up to several months?

Fill the chamber and the receptacle of the loosely inserted stopper with 70 % EtOH, add the Cover-slip and switch off the O2K.
- »MiPNet19.03 O2k-cleaning and ISS«
- communicated by »Leo Elettra« and »Grings Mateus«

Apr-15 Mo

Do you observe either a zero or low raw O₂ signal when starting a measurement?

Perform a test to evaluate whether this is related to an improperly positioned chamber or a faulty connection between the OroboPOS and the OroboPOS-Connector.
- »Oxygen signal«
- communicated by »Grings Mateus« and »Schmitt Sabine«

Apr-04 Th

Why is it important to check the H₂O₂ sensitivity when conducting an Amplex UltraRed (AmR) assay?

The sensitivity is influenced by experimental time, medium composition, added chemicals and sample, requiring multiple H₂O₂ calibrations throughout the measurement. After the initial AmR calibration, ensure that the sensitivity is within the recommended range.

- »MiPNet24.10 H2O2 flux analysis«, »Amplex UltraRed«
- communicated by »Grings Mateus« and »Schmitt Sabine«

Mar 2024

Mar-21 Th

How well do you handle your Hamilton syringes? What is a cleaning cycle?

"A syringe cleaning cycle consists of (1) filling an empty syringe with a washing solution while immersing [both the needle and] the base of the barrel into the washing solution, without fully removing the plunger, and (2) emptying the syringe into the waste beaker."
- »MiPNet19.14 SOP Hamilton microsyringes«
- communicated by »Baglivo Eleonora« and »Timon-Gomez Alba«

Mar-20 We

What if Alert: Error in titrations! appears in your O2 analysis template file?

The O2 analysis template recognizes discrepancies between the protocol selected in the first tab and the imported marks. Check the titration sequence and mark names against the protocol copied from the DLD file.
- »O₂ flux analysis«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«

Mar-07 Th

Which medium can be used for the instrumental background test?

When using dithionite, the instrumental background test should be performed in mitochondrial respiration medium MiR05, MiR05-Kit or MiR06; or alternatively, in a strong alkaline phosphate buffer (>100 mM; >pH 8), to avoid side reactions that might interfere with the test.
- »MiPNet14.06_Instrumental_O2_background«
- communicated by »Schmitt Sabine« and »Timon-Gomez Alba«

Mar-04 Mo

Do you observe an unstable O₂ slope negative following TIP2k titrations during an instrumental O₂ background test?

Incorrect placement of TIP2k Filter Papers can lead to liquid accumulation and convective oxygen backdiffusion. Check for proper placement by confirming they are fully in contact with the stopper receptacle.
- »Instrumental O₂ background«, »Unstable O₂ slope negative«
- communicated by »Grings Mateus« and »Baglivo Eleonora«

Feb 2024

Feb-22 Th

What to do if the glass of the O2k-chamber starts to become opaque?

"Turbidity on the glass wall may be caused by precipitated protein. Remove the chambers from the O2k and with the O2k-stirrers removed, immerse in a beaker filled with HCl (10 M) overnight under a hood."
-»MiPNet19.03_O2k-cleaning_and_ISS«
- communicated by »Cardoso Luiza« and »Leo Elettra«

Feb-08 Th

Can I use the same stock solutions for all reagents for different O2k-chamber volumes?

"For some reagents, the concentration of the stock solution differs between the 2.0-mL and the 0.5-mL chamber (O2k-sV-Module) to facilitate a precise volume titration."
-»MiPNet09.12 O2k-Titrations«
- communicated by »Timon-Gomez Alba« and »Leo Elettra«

Jan 2024

Jan-25 Th

How do I view and change the settings of a measurement/file in DatLab 8?

"O2k control (active) will always show the settings of the active recording [and allow changes], while O2k info (view) will show the settings of the recorded selected file."

-»MiPNet28.07 NextGen-O2k Series XB manual«; »MiPNet28.08 Oroboros O2k Series J manual«
- communicated by »Baglivo Eleonora« and »Grings Mateus«

Jan-11 Th

What are special features of the new SmartPOS (O2k-Series J and NextGen-O2k-Series XB)?

- "The electrical connections of the SmartPOS are protected thus there is no need to clean them."
- "The serial number of the SmartPOS is automatically recognized by the DatLab 8 software."

»MiPNet28.10 SmartPOS-service«
- communicated by »Grings Mateus« and »Baglivo Eleonora«

Jan-08 Mo

Do you need to export DatLab traces for your publication?

Save your graph as an editable picture using "Copy to clipboard" in DatLab 7.4 (.wmf) or "Save" in DatLab 8 (.svg).
- »Copy to clipboard in DatLab 7«, »Download DatLab 8«
- communicated by »Baglivo Eleonora« and »Grings Mateus«