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Difference between revisions of "O2k-Peltier Mitopedia"

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== Lower limit of experimental temperature ==
== Lower limit of experimental temperature ==


:::: From O2k-Series E there is a more efficient temperature management system in comparison compared to previous series. At a room temperature of 21.1 °C a stable Peltier power of about -83% was reached after about 1:0 h from setting the block  temperature from 37 °C to 0 °C. [[User:Capek Ondrej|Capek Ondrej]] 14:36, 4 February 2013 (CET)
::::* From O2k-Series E there is a more efficient temperature management system in comparison compared to previous series. At an ambient temperature of 21.1 °C, a stable Peltier power of about 83% reached 0 °C from 37 °C in about 1 h.


:::: The O2k is specified for a chamber temperature down to 4 °C at 25 °C room temperature. The use of lower temperatures is not within our O2k-specifications, therefore we did not evaluate the quality control parameters in these conditions. Please, take special precaution with your set temperature to 0.5 °C, you could have a damage in the chambers or any other component.
::::* For an ambient temperature of 25 °C,  the O2k-Chamber can reach lower temperatures down to 4 °C. The use of temperatures lower than 4 °C is not within our O2k-specifications, as such the quality control parameters under these conditions are not guaranteed. We recommend taking special precautions with a set temperature of 0.5 °C as it could potentially damage the O2k-Chambers or any other component.
::::* For experiments at 0 °C, the O2k can be exposed to ambient temperatures slightly above 0 °C (all the electronic components are evaluated under minimum temperatures of 0 °C, which should be avoided due to condensation concerns). For low room temperatures close to 0 °C a humidity control system should be used.
:::: With O2k-Series D a stable chamber temperature of 0 °C was obtained at a room temperature of ~14 °C.  The cooling power of the Peltier element reached 95% of its maximum capacity.


:::: To use lower temperatures than our O2k-specifications , see [[MiPNet06.05 Test Experiments on O2k-Specifications| test experiments on O2k-Specifications]]. Please note that power ambient temperature does not necessarily mean a "cold room". For a chamber temperature of 2 °C, an air conditioned room is sufficient.
::::* To use temperatures lower than our O2k-specifications , see [[MiPNet06.05 Test Experiments on O2k-Specifications| test experiments on O2k-Specifications]]. Please note that to reach a lower ambient temperature needed for experiments at 2 °C, a cold room is not necessarily required; an air-conditioned room should suffice.


:::: For experiments at 0 °C, the O2k can be exposed to ambient temperatures down to just above 0 °C (all the electronic components are rated at least down to 0 °C, but 0 °C room temperature should be avoided due to condensation concerns). For low room temperatures close to 0 °C, some means of humidity control should be used.
:::: With O2k-Series D a stable chamber temperature of 0 °C was obtained at a room temperature of about 14 °C.  The cooling power of the Peltier element reached 95% of maximum.


== Block temperature does not increase while heating with full power ==
== Troubleshooting ==
=== Block temperature does not increase while heating with full power ===
::::* '''Customer ID''': Stier A, [[FI_Turku_Nikinmaa M]]
::::* '''Customer ID''': Stier A, [[FI_Turku_Nikinmaa M]]
::::'''Question:''' 2020-04-22 (adapted from e-mail)
::::'''Question:'''  
Our « old » Oroboros (likely bought in 2004) seems to have a problem of temperature control (error message + T°C staying at RT despite a set T°C of 40°C). Following the instructions of [[DatLab_error_messages]]; I switched it off and on (likely > 10 times), tried with an older version of the software and different temperature (20 to 40°C), but nothing seems to fix the issue.
Our O2k (likely bought in 2004) seems to have a problem of temperature control (error message + T°C staying at RT despite a set T°C of 40°C). Following the instructions of [[DatLab_error_messages]]; I switched it off and on (likely > 10 times), tried with an older version of the software and different temperature (20 to 40°C), but nothing seems to fix the issue. Representative data is attached (2020-04-22).


[[File:Ticket-2020042210000011 O2k Peltier.png|center|700px]]
[[File:Ticket-2020042210000011 O2k Peltier.png|center|700px]]


::::'''Answer:''' See [[DatLab_error_messages#DatLab_error:_Average_block_temperature_over_past_10_min_has_not_reached_set_temperature|DatLab error: Average block temperature over past 10 min has not reached set temperature]] and [[DatLab_error_messages#DatLab_error:_Block_temperature_does_not_increase_while_heating_with_full_power|DatLab error: Block temperature does not increase while heating with full power]]. If the problem persists, the O2k-Peltier Temperature Control has an electronic defect and the O2k-Main Unit has to be sent to the electronics workshop of OROBOROS for service.
::::'''Answer:''' See [[DatLab_error_messages#DatLab_error:_Average_block_temperature_over_past_10_min_has_not_reached_set_temperature|DatLab error: Average block temperature over past 10 min has not reached set temperature]] and [[DatLab_error_messages#DatLab_error:_Block_temperature_does_not_increase_while_heating_with_full_power|DatLab error: Block temperature does not increase while heating with full power]]. If the problem persists, the O2k-Peltier Temperature Control has an electronic defect and the O2k-Main Unit has to be sent to the electronics workshop of OROBOROS for service.
::::* [[Category talk:O2k-Support]]





Revision as of 09:30, 13 May 2020


high-resolution terminology - matching measurements at high-resolution


O2k-Peltier Mitopedia

Description

O2k-Peltier Temperature Control: Built-in electronic thermostat controlling temperature for two O2k-Chambers in the range of 4 to 47 °C; ±0.002 °C (at room temperature). Continuous recording of the O2k-Copper Block temperature with DatLab. Temperature change from 20 to 30 °C within 15 min; cooling from 30 to 20 °C within 20 min. Integral component of the O2k-Main Unit. The electronic temperature control of the O2k replaced the conventional water jacket.


Reference: O2k-Peltier Temperature Control





MitoPedia O2k and high-resolution respirometry: O2k hardware 




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MitoPedia O2k and high-resolution respirometry: O2k-Open Support 



Lower limit of experimental temperature

  • From O2k-Series E there is a more efficient temperature management system in comparison compared to previous series. At an ambient temperature of 21.1 °C, a stable Peltier power of about 83% reached 0 °C from 37 °C in about 1 h.
  • For an ambient temperature of 25 °C, the O2k-Chamber can reach lower temperatures down to 4 °C. The use of temperatures lower than 4 °C is not within our O2k-specifications, as such the quality control parameters under these conditions are not guaranteed. We recommend taking special precautions with a set temperature of 0.5 °C as it could potentially damage the O2k-Chambers or any other component.
  • For experiments at 0 °C, the O2k can be exposed to ambient temperatures slightly above 0 °C (all the electronic components are evaluated under minimum temperatures of 0 °C, which should be avoided due to condensation concerns). For low room temperatures close to 0 °C a humidity control system should be used.
With O2k-Series D a stable chamber temperature of 0 °C was obtained at a room temperature of ~14 °C. The cooling power of the Peltier element reached 95% of its maximum capacity.
  • To use temperatures lower than our O2k-specifications , see test experiments on O2k-Specifications. Please note that to reach a lower ambient temperature needed for experiments at 2 °C, a cold room is not necessarily required; an air-conditioned room should suffice.


Troubleshooting

Block temperature does not increase while heating with full power

Question:

Our O2k (likely bought in 2004) seems to have a problem of temperature control (error message + T°C staying at RT despite a set T°C of 40°C). Following the instructions of DatLab_error_messages; I switched it off and on (likely > 10 times), tried with an older version of the software and different temperature (20 to 40°C), but nothing seems to fix the issue. Representative data is attached (2020-04-22).

Ticket-2020042210000011 O2k Peltier.png
Answer: See DatLab error: Average block temperature over past 10 min has not reached set temperature and DatLab error: Block temperature does not increase while heating with full power. If the problem persists, the O2k-Peltier Temperature Control has an electronic defect and the O2k-Main Unit has to be sent to the electronics workshop of OROBOROS for service.


MitoPedia O2k and high-resolution respirometry: O2k hardware